Ultra High Performance Liquid Chromatography and Its Applications

Ultra High Performance Liquid Chromatography and Its Applications

In addition, the book explores a variety of UHPLC applications designed to support research in such fields as pharmaceuticals, food safety, clinical medicine, and environmental science.

Author: Q. Alan Xu

Publisher: John Wiley & Sons

ISBN: 9781118533987

Category: Technology & Engineering

Page: 304

View: 115

Explores both the benefits and limitations of new UHPLCtechnology High performance liquid chromatography (HPLC) has been widelyused in analytical chemistry and biochemistry to separate,identify, and quantify compounds for decades. The science of liquidchromatography, however, was revolutionized a few years ago withthe advent of ultra-high performance liquid chromatography (UHPLC),which made it possible for researchers to analyze sample compoundswith greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and ItsApplications enables readers to maximize the performance ofUHPLC as well as develop UHPLC methods tailored to their particularresearch needs. Readers familiar with HPLC methods will learn howto transfer these methods to a UHPLC platform and vice versa. Inaddition, the book explores a variety of UHPLC applicationsdesigned to support research in such fields as pharmaceuticals,food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development andmethod transfer between HPLC and UHPLC platforms. It then examinespractical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drugmetabolism and pharmacokinetics studies for traditional Chinesemedicine. With its frank discussions of UHPLC's benefits and limitations,Ultra-High Performance Liquid Chromatography and ItsApplications equips analytical scientists with the skills andknowledge needed to take full advantage of this new separationtechnology.
Categories: Technology & Engineering

High Performance Liquid Chromatography

High Performance Liquid Chromatography

Ultrahigh-pressure reversed-phase liquid chromatography in packed capillary columns. Anal. Chem., 69(6), 983–989, 1997. [11] Xu QA Editor, Ultra-high Performance Liquid Chromatography and Its Application, Hoboken, NJ, John Wiley & Sons, ...

Author: Omar Al Sayed Omar

Publisher: Walter de Gruyter GmbH & Co KG

ISBN: 9783110529456

Category: Science

Page: 322

View: 188

The book provides an indispensable guide on how to use HPLC in pharmaceutical analysis and drug control. Following a hands-on approach, the authors give practical advices how to prepare stationary and mobile phases, choose a suitable detector and set up an HPLC analysis. The publication gives insight into the key pharmaceutical applications of HPLC and the latest requirements of the major regulatory agencies.
Categories: Science

Introduction to high performance liquid chromatography

Introduction to high performance liquid chromatography

Since the first edition of this book the major advances have been in column packings, where over ninety per cent of separations are now performed using chemically bonded microparticulate packings, and in instrumentation.

Author: R. Hamilton

Publisher: Springer Science & Business Media

ISBN: 9789400959385

Category: Science

Page: 248

View: 253

Since the first edition of this book the major advances have been in column packings, where over ninety per cent of separations are now performed using chemically bonded microparticulate packings, and in instrumentation. The use of microprocessor control has brought about a rationalization of mobile phase delivery systems and in detectors, the introduction of electrochemical and spectrophotometric detection other than in the ultra-violet region, has widened the field of applications and the sensitivity of the technique. The use of ion pair chromatography has increased at the expense of ion-exchange and this together with the improvements in detectors has greatly increased the appli cation of the technique in the biomedical field. These advances are described together with the established methods to enable the beginner to carry out a satisfactory separation and to gain the experience necessary for the full exploitation of the technique. R. J. Hamilton P. A. Sewell Liverpool,1981 1 Introduction to high performance liquid chromatography 1. 1 Introduction Chromatography in its many forms is widely used as a separative and an analytical technique. Gas chromatography since its introduction by James and Martin [1] has been pre-eminent in the field. Uquid chromatography in the of paper, thin-layer, ion-exchange, and exclusion (gel permeation and gel form filtration) chromatography had not been able to achieve the same success, mainly because of the poor efficiences and the long analysis times arising from the low mobile phase flow rates.
Categories: Science

Ultra Performance Liquid Chromatography Mass Spectrometry

Ultra Performance Liquid Chromatography Mass Spectrometry

Application of ultra-high-pressure liquid chromatography-tandem mass spectrometry to the determination of multi-class pesticides in environmental and wastewater samples study of matrix effects. J. Chromatogr. A 1216:1410–1420.

Author: Mu Naushad

Publisher: CRC Press

ISBN: 9781466591547

Category: Science

Page: 480

View: 435

Due to its high sensitivity and selectivity, liquid chromatography–mass spectrometry (LC–MS) is a powerful technique. It is used for various applications, often involving the detection and identification of chemicals in a complex mixture. Ultra Performance Liquid Chromatography Mass Spectrometry: Evaluation and Applications in Food Analysis presents a unique collection of up-to-date UPLC-MS/MS methods for the separation and quantitative determination of components, contaminants, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. The book begins with an overview of the history, principles, and advancement of chromatography. It discusses the use of UHPLC techniques in food metablomics, approaches for analysis of foodborne carcinogens, and details of UPLC-MS techniques used for the separation and determination of capsaicinoids. Chapters describe the analysis of contaminants in food, including pesticides, aflatoxin, perfluorochemicals, and acrylamide, as well as potentially carcinogenic heterocyclic amines in cooked foods. The book covers food analysis for beneficial compounds, such as the determination of folate, vitamin content analysis, applications for avocado metabolite studies, virgin olive oil component analysis, lactose determination in milk, and analysis of minor components of cocoa and phenolic compounds in fruits and vegetables. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.
Categories: Science

Cumulated Index Medicus

Cumulated Index Medicus

Cheng FC , et al . high - performance liquid chromatography with Apr ; 24 ( 4 ) : 324-9 J Chromatogr 1993 Aug 11 ; 617 ( 2 ) : 227-32 fluorescence detection . Kelly MJ , et al . J Chromatogr 1993 Evaluation of the REMEDi drug profiling ...

Author:

Publisher:

ISBN: OSU:32436010002382

Category: Medicine

Page: 1500

View: 125

Categories: Medicine

High performance Liquid Chromatography

High performance Liquid Chromatography

In this collection, the authors discuss the way in which it is possible to detect the low concentrations at which toxic compounds and metabolites are present in specimens due to the huge development of chromatographic techniques.

Author: Ivan Lucero

Publisher:

ISBN: 1536135437

Category:

Page:

View: 497

Categories:

Liquid Chromatography

Liquid Chromatography

Applications Salvatore Fanali, Paul R. Haddad, Colin Poole, Marja-Liisa Riekkola ... chemometric analysis for the quality evaluation of three different frankincenses by ultra high performance liquid chromatography and quadrupole time of ...

Author: Salvatore Fanali

Publisher: Elsevier

ISBN: 9780128093443

Category: Science

Page: 838

View: 802

Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making
Categories: Science

Organic Trace Analysis

Organic Trace Analysis

Ultra-high performance liquid chromatography and its application. Hoboken, NJ: John Wiley & Sons, Inc., 2013. ... Zuo Y. High-performance liquid chromatography (HPLC): principles, practices and procedures. Hauppauge, NY: Nova Science ...

Author: Reinhard Nießner

Publisher: Walter de Gruyter GmbH & Co KG

ISBN: 9783110441154

Category: Science

Page: 369

View: 972

"Organic Trace Analysis" presents the basics of trace analysis, from sample preparation to the measurement: Students are introduced to statistical evaluation, quality control technologies, sampling and preparation of organic traces, as well as to enrichment and separation of samples. Spectroscopic techniques as chromatography, capillary electrophoresis, mass spectrometry, and receptor-based bioanalysis are presented in detail.
Categories: Science

Fast Liquid Chromatography Mass Spectrometry Methods in Food and Environmental Analysis

Fast Liquid Chromatography   Mass Spectrometry Methods in Food and Environmental Analysis

'The potential of shell particles in fast liquid chromatography', in Xu, Q.A. (ed.), Ultra-High Performance Liquid Chromatogaphy and its Applications, John Wiley & Sons, Hoboken, New Jersey, pp. 133–167. 9. Horvath, C. and Lipsky, ...

Author: Oscar Núñez

Publisher: World Scientific

ISBN: 9781783264957

Category: Science

Page: 624

View: 412

There is a growing need for high-throughput separations in food and environmental research that are able to cope with the analysis of a large number of compounds in very complex matrices. Whereas the most common approach for solving many analytical problems has often been high-performance liquid chromatography (HPLC), the recent use of fast or ultra-fast chromatographic methods for environmental and food analysis has increased the overall sample throughput and laboratory efficiency without loss (and even with an improvement) in the resolution obtained by conventional HPLC systems. This book brings together researchers at the top of their field from across the world to discuss and analyze recent advances in fast liquid chromatography–mass spectrometry (LC–MS) methods in food and environmental analysis. First, the most novel approaches to achieve fast and ultra-fast methods as well as the use of alternative and complementary stationary phases are described. Then, recent advances in fast LC–MS methods are addressed, focusing on novel treatment procedures coupled with LC–MS, new ionization sources, high-resolution mass spectrometry, and the problematic confirmation and quantification aspects in mass spectrometry. Finally, relevant LC–MS applications in food and environmental analysis such as the analysis of pesticides, mycotoxins, food packaging contaminants, perfluorinated compounds and polyphenolic compounds are described. The scope of the book is intentionally broad and is aimed at worldwide analytical laboratories working in food and environmental applications as well as researchers in universities worldwide. Contents: Fast Liquid Chromatography Advances:UHPLC Separations Using Sub-2 μm Particle Size Columns (Julie Schappler, Jean-Luc Veuthey and Davy Guillarme)Core-Shell Column Technology in Fast Liquid Chromatography (Oscar Núñez and Héctor Gallart-Ayala)Monolithic Columns in Fast Liquid Chromatography (Takeshi Hara, Oscar Núñez, Tohru Ikegami and Nobuo Tanaka)High-Temperature Liquid Chromatography (Thorsten Teutenberg)Hydrophilic Interaction Liquid Chromatography (HILIC) and Perfluorinated Stationary Phases (Cristina C Jacob, Héctor Gallart-Ayala and Gonçalo Gamboa da Costa)Advances in Fast Liquid Chromatography–Mass Spectrometry Methods:On-Line Sample Pre-Treatment Procedures Applied to LC–MS (Tony Edge and Joseph Herman)Ambient Mass Spectrometry: Food and Environmental Applications (Tiina J Kauppila and Anu Vaikkinen)Liquid Chromatography–High–Resolution Mass Spectrometry in Environmental and Food Analysis (Paolo Lucci and Claudia P B Martins)Liquid Chromatography–Mass Spectrometry: Quantification and Confirmation Aspects (Jaume Aceña, Daniel Rivas, Bozo Zonja, Sandra Pérez and Damià Barceló)Relevant LC–MS Applications in Food and Environmental Analysis:Multiresidue Analysis of Pesticides: LC–MS/MS versus LC–HRMS (Juan V Sancho and María Ibáñez)Food-Packaging Contaminants (Silvia Lacorte, Montse Cortina, Albert Guart and Antonio Borrell)Liquid Chromatography–Mass Spectrometry for the Analysis of Perfluorinated Compounds in Water Samples (Marianna Rusconi, Stefano Polesello and Sara Valsecchi)Determination of Phenolic Compounds in Food Matrices: Application to Characterization and Authentication (Javier Saurina and Sonia Sentellas)Liquid Chromatography–Mass Spectrometric Analysis of Mycotoxins in Food (Veronica M T Lattanzio and Angelo Visconti) Readership: Scientists or students in mass spectrometry, chemists, biologists, and analysts. Keywords:Mass Spectrometry;Fast Liquid Chromatography;Food Analysis;Environmental Analysis
Categories: Science

Advances in Chromatography

Advances in Chromatography

Volume 20 High-Performance Liquid Chromatography and Its Application to Protein Chemistry Milton T.W.Hearn ... of Polymers of Ultrahigh Molecular Weight J.Calvin Giddings Volume 21 High-Performance Liquid Chromatography/Mass ...

Author: Phyllis R. Brown

Publisher: CRC Press

ISBN: 9780824747268

Category: Science

Page: 448

View: 756

Illustrating developments in separation science and chromatographic analysis, this volume investigates trends in chemometrics, proteomics, column technology, and element-selective detection for pharmaceutical, medical, industrial and environmental applications.
Categories: Science