The AGT Cytogenetics Laboratory Manual

The AGT Cytogenetics Laboratory Manual

Reviews for the third edition: "A collection of protocols and explanations for cytogenetic techniques from the common to the peculiar." "An excellent book. Looking forward to an updated version."

Author: Marilyn S. Arsham

Publisher: John Wiley & Sons

ISBN: 9781119061175

Category: Science

Page: 1218

View: 190

Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.
Categories: Science

The AGT Cytogenetics Laboratory Manual

The AGT Cytogenetics Laboratory Manual

Author: Association of Genetic Technologists

Publisher: Lippincott Williams & Wilkins

ISBN: NWU:35558004885329

Category: Science

Page: 666

View: 614

Revised and expanded to cover the most advanced instrumentation and techniques in the field of cytogenetics, this third edition includes: an expanded FISH chapter; information and protocols for breakage studies; coverage of computer imaging, regulation, and the molecular aspects of leukemia; and chromosone spreading. Over 200 step-by-step protocols are also presented throughout the text.
Categories: Science

Emery s Elements of Medical Genetics

Emery s Elements of Medical Genetics

Barch MJ , Knutsen T , Spurbeck J L ( eds ) 1997 The AGT cytogenetics
laboratory manual , 3rd edn . Lippincott - Raven , Philadelphia A large
multiauthor laboratory handbook produced by the Association of Genetic
Technologists . Gersen SL ...

Author: Robert F. Mueller

Publisher:

ISBN: UOM:39015053139039

Category: Medical

Page: 372

View: 902

Today's medical student needs to understand the principles of genetics rather than accumulate detailed facts. This text explains the essential themes of medical genetics whilst remaining in control of the developments in this subject.
Categories: Medical

Interferon mediated Block in the Cell Cycle and Alteration of Integrin Expression in an in Vitro Model of Sj gren s Syndrome

Interferon mediated Block in the Cell Cycle and Alteration of Integrin Expression in an in Vitro Model of Sj  gren s Syndrome

In : Barch MJ , Knutsen , T , Sparbeck , JL The AGT Cytogenetics Laboratory
Manual . 3rd ed . Philadelphia , Pa : Lippincott - Ranen ; 1997 : 190 . 6 . Strober
W . Current Protocols in Immunology . New York , NY : Greene Publishing and
Wiley ...

Author: Patrick J. Daniels

Publisher:

ISBN: UOM:39015042253776

Category: Salivary glands

Page: 154

View: 480

The sialoadenitis seen in Sjögren's syndrome, an idiopathic, autoimmune exocrinopathy, is characterized by lymphocytic infiltration, acinar cell atrophy, and diminished salivary flow. Increased expressions of laminin, a laminin receptor, and cytokines are also noted. Several in vivo characteristics of the sialoadenitis are also evident in a cytokine-treated salivary gland ductal epithelial cell line. To elucidate mechanisms of salivary gland pathology, the effects of two cytokines, interferon gamma and tumor necrosis factor alpha, on cell proliferation and expressions of basement membrane proteins and alpha-3 integrin were evaluated in cultured salivary gland cells. In cytokine-treated cell monolayers, immunoprecipitation, immunoperoxidase, and Western Blot analysis demonstrated a moderate intracellular accumulation of an immature laminin product, but not fibronectin or collagen IV, concurrent with decreased cell proliferation. Results from RNase Protection assays suggested that the laminin accumulation was unlikely due to increased laminin beta chain gene expression. Furthermore, a significant reduction of glyceraldehyde-3-phosphate dehydrogenase expression was noted with prolonged cytokine treatment, suggesting metabolic defects. To explore the effects of cytokines on acinar cell pathology, cells were grown on Matrigel, where they formed acini with polarized nuclei. Cytokine treatment arrested cells in G1 phase of the cell cycle, as evaluated by flow cytometry, which preceded significant morphological changes and decreased viability. By immunoprecipitation, an altered form of alpha-3 integrin was evident in cultured acinar cells treated with cytokines for prolonged periods, but not in untreated cells. Cytoklines caused no significant changes in laminin expression in acinar cells. From this study, it was evident that the combination of interferon gamma and tumor necrosis factor alpha resulted in a block in G1 phase for acinar cells. This cell cycle arrest occurred prior to accumulation of the alpha-3 integrin variant or significant degenerative cellular changes. Information from the present and previous studies suggest that cytokines may alter adhesion and block cell cycle progression in acinar cells in Sjögren's syndrome sialoadenitis. Further studies may help elucidate how these cytokine-mediated cellular changes contribute to acinar cell death.
Categories: Salivary glands

Annals of the Academy of Medicine Singapore

Annals of the Academy of Medicine  Singapore

Lawce HJ , Brown MG . Cytogenetics : an overview . In : Barch MJ , Knutsen T ,
Spurbeck JL , editors . The AGT Cytogenetics Laboratory Manual . 3rd ed . New
York : Lippincott - Raven Press , 1997 : 19-75 . 3. Claussen U , Michel S , Muhlig
P ...

Author: Academy of Medicine (Singapore)

Publisher:

ISBN: UCLA:L0090327016

Category:

Page:

View: 624

Categories:

New Books in the Veterinary Medicine Library

New Books in the Veterinary Medicine Library

W353 1984. Laboratory studies of chick , pig , and frog embryos : guide and atlas
of vertebrate embryology . Minneapolis : Burgess Pub . Co. , 1984 . ... Academy of
Sciences , 1991 . RB44.A38 1997. The AGT cytogenetics laboratory manual .

Author: Ohio State University. Libraries. Veterinary Medicine Library

Publisher:

ISBN: OSU:32435058072281

Category: Veterinary medicine

Page:

View: 572

Categories: Veterinary medicine

Alternative Splicing Variation of Chicken Telomerase Reverse Transcriptase

Alternative Splicing Variation of Chicken Telomerase Reverse Transcriptase

Thompson FH ( 1997 ) Cytogenetic methods and findings in human solid tumors .
In : MJ Barch , T Knutsen , JL Spurbeck , eds . The AGT Cytogenetics Laboratory
Manual , Third Edition . Philadelphia : Lippincott - Raven Publishers , pp ...

Author: Hong Chang

Publisher:

ISBN: UCAL:X76675

Category:

Page: 316

View: 667

Categories:

Murine RAD50S Reveals the Importance of DNA Damage Responses in Homeostasis

Murine RAD50S Reveals the Importance of DNA Damage Responses in Homeostasis

Roulston , D . , and Le Beau , M . M . ( 1997 ) . Cytogenetic analysis of
hematologic malignant disease . In The AGT Cytogenetics Laboratory Manual , M
. J . Barch , T . Knutsen , and J . Spurbeck , eds . ( Philadelphia , PA , Lippincott -
Raven ) ...

Author: Carla F. Bender

Publisher:

ISBN: WISC:89085269736

Category:

Page: 205

View: 260

Categories:

Characterization of Microsatellite Loci in Three Owl Monkey Species Aotus Azarai A Lemurinus A Nancymaae

Characterization of Microsatellite Loci in Three Owl Monkey Species  Aotus Azarai  A  Lemurinus  A  Nancymaae

In : The AGT Cytogenetics Laboratory Manual ( eds . Barch MJ , Knutsen T ,
Spurbeck JL ) , pp . 173-197 . Lippincott - Raven Publishers , Pennsylvania .
Schneider S , Roessli D , Excoffier L ( 2000 ) Arlequin : A software for population
genetics ...

Author: Jennie Lau

Publisher:

ISBN: UCSD:31822009430299

Category:

Page: 74

View: 903

Categories:

Journal of the National Cancer Institute

Journal of the National Cancer Institute

( 14 ) Barch M , Knutsen T , Spurbeck J. The AGT cytogenetics laboratory manual
. 3rd ed . Philadelphia ( PA ) : Lippincott - Raven ; 1997 . ( 15 ) Marineau C ,
Aubry M , Julien JP , Rouleau GA . Dinucleotide repeat polymorphism at the
D22S264 ...

Author:

Publisher:

ISBN: COLUMBIA:HR01790765

Category: Cancer

Page:

View: 173

Categories: Cancer

The Science of Laboratory Diagnosis

The Science of Laboratory Diagnosis

In The AGT Cytogenetics Laboratory Manual , 3rd edn , Barch MJ , Knutsen , T ,
Spurbeck JL ( eds ) . 1997 : Lippincott - Raven , New York . Holmquist G , Gray M
, Porter T , Jordan J . Characterization of Giemsa dark and light band DNA .

Author: David Burnett

Publisher: Wiley-Blackwell

ISBN: UOM:39015062492643

Category: Medical

Page: 542

View: 301

This book provides a concise description of all common laboratory tests available in medical practice with notes on their application. The accuracy of each test, the historical background to the adoption of various tests and their effectiveness in diagnosis. The text benefits from the use of clear headings, tables, flowcharts and pathology slides, the majority of which are in full color.
Categories: Medical

Cytobios

Cytobios

Molecular cytogenetics : definitions , clinical aspects , and protocols . In The AGT
Cytogenetics Laboratory Manual . Third editn . Edited by M . J . Barch , T .
Knutsen and J . L . Spurbeck . Lippincott - Raven Publishers , Philadelphia .
SCHRÖCK ...

Author:

Publisher:

ISBN: UOM:39015046057009

Category: Cytology

Page:

View: 305

Categories: Cytology

Emery s Elements of Medical Genetics

Emery s Elements of Medical Genetics

Barch M J , Knutsen T , Spurbeck J L ( eds ) 1997 The AGT cytogenetics
laboratory manual , 3rd edn . Lippincott - Raven , Philadelphia A large
multiauthor laboratory handbook produced by the Association of Genetic
Technologists . Gersen ...

Author: Peter D. Turnpenny

Publisher: Churchill Livingstone

ISBN: UOM:39015060554188

Category: Medical

Page: 443

View: 137

Covers both the scientific basis and clinical applications of genetics. For this new edition, the content has been thoroughly updated to keep abreast of this fast-changing area, including molecular genetics, DNA technology, and ethics. It also features a new self-testing section, new clinical examples, and updated and improved clinical photographs. Offers a comprehensive, clinically relevant overview of the field with clear, concise text. Features "Elements" boxes at the end of the chapter that provide a bulleted chapter overview for the reader. Depicts rare diseases with a genetic basis in high-quality color and black-and-white photographs.
Categories: Medical

Proceedings of the National Academy of Sciences of the United States of America

Proceedings of the National Academy of Sciences of the United States of America

Barch , M. J. , Knutsen , T. & Spurbeck , J. L. The AGT Cytogenetics Laboratory
Manual ( 1997 ) ( Lippincott - Raven , Philadelphia ) . 16. Hahn , W. C. , Stewart ,
S. A. , Brooks , M. W. , York , S. G. , Eaton , E. , Kurachi , A. , Beijersbergen , R. L.
 ...

Author: National Academy of Sciences (U.S.)

Publisher:

ISBN: WISC:89099177065

Category: Science

Page:

View: 662

Categories: Science

The Cumulative Book Index

The Cumulative Book Index

Author:

Publisher:

ISBN: UOM:39015058373930

Category: American literature

Page:

View: 690

A world list of books in the English language.
Categories: American literature

The Journal of Cell Biology

The Journal of Cell Biology

Glycine residues appear to be evolutionarily conserved for their ability to inhibit
aggregation . Structure . 13 : 1143 - 1151 . Priest , J . H . 1997 . General cell
culture principles and fibroblast culture . In The AGT Cytogenetics Laboratory
Manual .

Author:

Publisher:

ISBN: UOM:39015058997514

Category: Cytology

Page:

View: 489

No. 2, pt. 2 of November issue each year from v. 19 (1963)-47 (1970) and v. 55 (1972)- contain the Abstracts of papers presented at the Annual Meeting of the American Society for Cell Biology, 3d (1963)-10th (1970) and 12th (1972)-
Categories: Cytology