Reviews for the third edition: "A collection of protocols and explanations for cytogenetic techniques from the common to the peculiar." "An excellent book. Looking forward to an updated version."
Author: Marilyn S. Arsham
Publisher: John Wiley & Sons
Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.
5,600 Flash Cards. Q & A FlashCards, Ebooks, Textbooks, Courses, Books Simplified as FlashCards by Powell Publications. Very effective study tools especially when you only have a limited amount of time.
Author: Association of Genetic TechnologistsPublish On: 1997
Author: Association of Genetic Technologists
Publisher: Lippincott Williams & Wilkins
Revised and expanded to cover the most advanced instrumentation and techniques in the field of cytogenetics, this third edition includes: an expanded FISH chapter; information and protocols for breakage studies; coverage of computer imaging, regulation, and the molecular aspects of leukemia; and chromosone spreading. Over 200 step-by-step protocols are also presented throughout the text.
Barch MJ , Knutsen T , Spurbeck J L ( eds ) 1997 The AGT cytogenetics laboratory manual , 3rd edn . Lippincott - Raven , Philadelphia A large
multiauthor laboratory handbook produced by the Association of Genetic
Technologists . Gersen SL ...
Author: Robert F. Mueller
Today's medical student needs to understand the principles of genetics rather than accumulate detailed facts. This text explains the essential themes of medical genetics whilst remaining in control of the developments in this subject.
In : Barch MJ , Knutsen , T , Sparbeck , JL The AGT Cytogenetics Laboratory Manual . 3rd ed . Philadelphia , Pa : Lippincott - Ranen ; 1997 : 190 . 6 . Strober
W . Current Protocols in Immunology . New York , NY : Greene Publishing and
Author: Patrick J. Daniels
Category: Salivary glands
The sialoadenitis seen in Sjögren's syndrome, an idiopathic, autoimmune exocrinopathy, is characterized by lymphocytic infiltration, acinar cell atrophy, and diminished salivary flow. Increased expressions of laminin, a laminin receptor, and cytokines are also noted. Several in vivo characteristics of the sialoadenitis are also evident in a cytokine-treated salivary gland ductal epithelial cell line. To elucidate mechanisms of salivary gland pathology, the effects of two cytokines, interferon gamma and tumor necrosis factor alpha, on cell proliferation and expressions of basement membrane proteins and alpha-3 integrin were evaluated in cultured salivary gland cells. In cytokine-treated cell monolayers, immunoprecipitation, immunoperoxidase, and Western Blot analysis demonstrated a moderate intracellular accumulation of an immature laminin product, but not fibronectin or collagen IV, concurrent with decreased cell proliferation. Results from RNase Protection assays suggested that the laminin accumulation was unlikely due to increased laminin beta chain gene expression. Furthermore, a significant reduction of glyceraldehyde-3-phosphate dehydrogenase expression was noted with prolonged cytokine treatment, suggesting metabolic defects. To explore the effects of cytokines on acinar cell pathology, cells were grown on Matrigel, where they formed acini with polarized nuclei. Cytokine treatment arrested cells in G1 phase of the cell cycle, as evaluated by flow cytometry, which preceded significant morphological changes and decreased viability. By immunoprecipitation, an altered form of alpha-3 integrin was evident in cultured acinar cells treated with cytokines for prolonged periods, but not in untreated cells. Cytoklines caused no significant changes in laminin expression in acinar cells. From this study, it was evident that the combination of interferon gamma and tumor necrosis factor alpha resulted in a block in G1 phase for acinar cells. This cell cycle arrest occurred prior to accumulation of the alpha-3 integrin variant or significant degenerative cellular changes. Information from the present and previous studies suggest that cytokines may alter adhesion and block cell cycle progression in acinar cells in Sjögren's syndrome sialoadenitis. Further studies may help elucidate how these cytokine-mediated cellular changes contribute to acinar cell death.
Author: Academy of Medicine (Singapore)Publish On: 2004
Lawce HJ , Brown MG . Cytogenetics : an overview . In : Barch MJ , Knutsen T ,
Spurbeck JL , editors . The AGT Cytogenetics Laboratory Manual . 3rd ed . New
York : Lippincott - Raven Press , 1997 : 19-75 . 3. Claussen U , Michel S , Muhlig
Roulston , D . , and Le Beau , M . M . ( 1997 ) . Cytogenetic analysis of
hematologic malignant disease . In The AGT Cytogenetics Laboratory Manual , M
. J . Barch , T . Knutsen , and J . Spurbeck , eds . ( Philadelphia , PA , Lippincott -
Raven ) ...
( 14 ) Barch M , Knutsen T , Spurbeck J. The AGT cytogenetics laboratory manual
. 3rd ed . Philadelphia ( PA ) : Lippincott - Raven ; 1997 . ( 15 ) Marineau C ,
Aubry M , Julien JP , Rouleau GA . Dinucleotide repeat polymorphism at the
In The AGT Cytogenetics Laboratory Manual , 3rd edn , Barch MJ , Knutsen , T ,
Spurbeck JL ( eds ) . 1997 : Lippincott - Raven , New York . Holmquist G , Gray M
, Porter T , Jordan J . Characterization of Giemsa dark and light band DNA .
Author: David Burnett
This book provides a concise description of all common laboratory tests available in medical practice with notes on their application. The accuracy of each test, the historical background to the adoption of various tests and their effectiveness in diagnosis. The text benefits from the use of clear headings, tables, flowcharts and pathology slides, the majority of which are in full color.
Molecular cytogenetics : definitions , clinical aspects , and protocols . In The AGT Cytogenetics Laboratory Manual . Third editn . Edited by M . J . Barch , T .
Knutsen and J . L . Spurbeck . Lippincott - Raven Publishers , Philadelphia .
Barch M J , Knutsen T , Spurbeck J L ( eds ) 1997 The AGT cytogenetics laboratory manual , 3rd edn . Lippincott - Raven , Philadelphia A large
multiauthor laboratory handbook produced by the Association of Genetic
Technologists . Gersen ...
Author: Peter D. Turnpenny
Publisher: Churchill Livingstone
Covers both the scientific basis and clinical applications of genetics. For this new edition, the content has been thoroughly updated to keep abreast of this fast-changing area, including molecular genetics, DNA technology, and ethics. It also features a new self-testing section, new clinical examples, and updated and improved clinical photographs. Offers a comprehensive, clinically relevant overview of the field with clear, concise text. Features "Elements" boxes at the end of the chapter that provide a bulleted chapter overview for the reader. Depicts rare diseases with a genetic basis in high-quality color and black-and-white photographs.
Author: National Academy of Sciences (U.S.)Publish On: 2005
Barch , M. J. , Knutsen , T. & Spurbeck , J. L. The AGT Cytogenetics Laboratory Manual ( 1997 ) ( Lippincott - Raven , Philadelphia ) . 16. Hahn , W. C. , Stewart ,
S. A. , Brooks , M. W. , York , S. G. , Eaton , E. , Kurachi , A. , Beijersbergen , R. L.
Glycine residues appear to be evolutionarily conserved for their ability to inhibit
aggregation . Structure . 13 : 1143 - 1151 . Priest , J . H . 1997 . General cell
culture principles and fibroblast culture . In The AGT Cytogenetics Laboratory Manual .
No. 2, pt. 2 of November issue each year from v. 19 (1963)-47 (1970) and v. 55 (1972)- contain the Abstracts of papers presented at the Annual Meeting of the American Society for Cell Biology, 3d (1963)-10th (1970) and 12th (1972)-