Plant Protoplasts and Genetic Engineering II

Plant Protoplasts and Genetic Engineering II

The present volume, Plant Protoplasts and Genetic Engineering II, deals with some of the newer techniques such as microinjections, elec­ trofusion, flow cytometry, uptake and integration of DNA, nuclei, iso­ lated chromosomes by plant ...

Author: Y. P. S. Bajaj

Publisher: Springer

ISBN: 3642744567

Category: Technology & Engineering

Page: 499

View: 425

Genetic engineering through DNA recombinants and the in vitro manipulation of isolated protoplasts has recently attracted much atten tion in agricultural biotechnology, and has greatly advanced during the last 5 years. In an earlier book, Plant Protoplasts and Genetic Engineer ing I, methods for the isolation, fusion and culture of protoplasts were reviewed and the regeneration of complete plants from isolated pro toplasts of rice, potato, soybean, linseed, cabbage, chicory, lettuce, but terbur, orchids, citrus and some other tree species, and interspecific and intergeneric somatic hybrids in Lycopersicon, Petunia, Nicotiana, Solanum, Glycine, Citrus, Brassica, Medicago and Trifolium spp. were discussed. The present volume, Plant Protoplasts and Genetic Engineering II, deals with some of the newer techniques such as microinjections, elec trofusion, flow cytometry, uptake and integration of DNA, nuclei, iso lated chromosomes by plant protoplasts and the subsequent regeneration of transgenic plants. The literature on the DNA recombinants and genetic transformation, both Agrobacterium-mediated and direct gene transfer in agricultural crops and trees, such as poplars, is reviewed, and the uses of cytoplasts and miniprotoplasts in genetic manipulation are highlighted.
Categories: Technology & Engineering

Plant Protoplasts and Genetic Engineering I

Plant Protoplasts and Genetic Engineering I

The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic ...

Author: Y. P. S. Bajaj

Publisher: Springer

ISBN: 3642736165

Category: Technology & Engineering

Page: 444

View: 726

Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-· toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean prepara tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Inter national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, in corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite.
Categories: Technology & Engineering

Plant Protoplasts and Genetic Engineering VI

Plant Protoplasts and Genetic Engineering VI

This volume comprising 28 chapters on the in vitro manipulation of plant protoplasts contributed by inter- national experts deals with the isolation, fusion, culture, immobilization, cryopreservation and ultrastructural studies on ...

Author: Y.P.S Bajaj

Publisher: Springer Science & Business Media

ISBN: 9783642578403

Category: Technology & Engineering

Page: 313

View: 241

This volume comprising 28 chapters on the in vitro manipulation of plant protoplasts contributed by inter- national experts deals with the isolation, fusion, culture, immobilization, cryopreservation and ultrastructural studies on protoplasts and the regeneration of somatic hybrids and cybrids.
Categories: Technology & Engineering

Plant Protoplasts and Genetic Engineering IV

Plant Protoplasts and Genetic Engineering IV

In continuation of Volumes 8, 9, and 22 on in vitro manipulation of plant protplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Actinidia, Amoracia, Beta, ...

Author: Y. P. S. Bajaj

Publisher: Springer

ISBN: 3642780393

Category: Technology & Engineering

Page: 390

View: 941

In continuation of Volumes 8, 9, and 22 on in vitro manipulation of plant protplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Actinidia, Amoracia, Beta, Brassica, Cicer, Citrus, Cucumis, Duboisia, Fragaria, Glycine, Ipomoea, Lactuca, Lotus, Lycopersicon, Manihot, Medicago, Nicotiana, Petunia, Phaseolus, Pisum, Prunus, Psophocarpus, Saccharum, Solanum, Sorghum, Stylosanthes, and Vitis. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.
Categories: Technology & Engineering

Plant Protoplasts and Genetic Engineering VII

Plant Protoplasts and Genetic Engineering VII

This volume is of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general plant biotechnology.

Author: Y. P. S. Bajaj

Publisher: Springer Science & Business Media

ISBN: 9783662093689

Category: Technology & Engineering

Page: 321

View: 318

Twenty-seven chapters deal with the regeneration of plants from protoplasts and genetic transformation in various species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physocomitrella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. This volume is of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general plant biotechnology.
Categories: Technology & Engineering

Plant Protoplasts and Genetic Engineering V

Plant Protoplasts and Genetic Engineering V

In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, ...

Author: Professor Dr. Y. P. S. Bajaj

Publisher: Springer Science & Business Media

ISBN: 9783662093665

Category: Technology & Engineering

Page: 414

View: 192

In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.
Categories: Technology & Engineering

Plant Protoplasts and Genetic Engineering III

Plant Protoplasts and Genetic Engineering III

In continuation of Volumes 8 and 9 (1989) on in vitro manipulation of plant protoplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Agrostis, Arabidopsis, Atropa, ...

Author: Y. P. S. Bajaj

Publisher: Springer Science & Business Media

ISBN: 9783642780066

Category: Technology & Engineering

Page: 332

View: 175

In continuation of Volumes 8 and 9 (1989) on in vitro manipulation of plant protoplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Agrostis, Arabidopsis, Atropa, Brassica, Catharanthus, Datura, Cucumis, Daucus, Digitalis, Duboisia, Eustoma, Festuca, Helianthus, Hordeum, Kalanchoe, Linum, Lobelia, Lolium, Lotus, Lycium, Lycopersicum, Mentha, Nicotiana, Pelargonium, Pisum, Pyrus, Salvia, Scopolia, and Solanum.These studies reflect the far reaching implications of protoplast technologyin genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.
Categories: Technology & Engineering

Plant Protoplasts and Genetic Engineering I

Plant Protoplasts and Genetic Engineering I

This volume comprising 28 chapters on the in vitro manipulation of plant protoplasts contributed by inter- national experts deals with the isolation, fusion, culture, immobilization, cryopreservation and ultrastructural studies on ...

Author: Y. P. S. Bajaj

Publisher: Springer

ISBN: UOM:39015022551207

Category: Science

Page: 444

View: 274

This volume comprising 28 chapters on the in vitro manipulation of plant protoplasts contributed by inter- national experts deals with the isolation, fusion, culture, immobilization, cryopreservation and ultrastructural studies on protoplasts and the regeneration of somatic hybrids and cybrids.
Categories: Science

Plant Protoplasts and Genetic Engineering IV

Plant Protoplasts and Genetic Engineering IV

In continuation of Volumes 8, 9, and 22 on in vitro manipulation of plant protplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Actinidia, Amoracia, Beta, ...

Author: Y. P. S. Bajaj

Publisher: Springer

ISBN: 3540563849

Category: Technology & Engineering

Page: 390

View: 359

In continuation of Volumes 8, 9, and 22 on in vitro manipulation of plant protplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Actinidia, Amoracia, Beta, Brassica, Cicer, Citrus, Cucumis, Duboisia, Fragaria, Glycine, Ipomoea, Lactuca, Lotus, Lycopersicon, Manihot, Medicago, Nicotiana, Petunia, Phaseolus, Pisum, Prunus, Psophocarpus, Saccharum, Solanum, Sorghum, Stylosanthes, and Vitis. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.
Categories: Technology & Engineering

Plant Protoplasts and Genetic Engineering II

Plant Protoplasts and Genetic Engineering II

toplasts were taken up into plant protoplast cytoplasm by endocytosis with
vesicles, since two limiting membranes were seen in electron micrographs. In the
long term, these vesicles may interact with lysosomes, resulting in breakdown of
the ...

Author: Y. P. S. Bajaj

Publisher: Springer Science & Business Media

ISBN: 9783642744549

Category: Technology & Engineering

Page: 499

View: 961

Categories: Technology & Engineering

Protoplast Fusion

Protoplast Fusion

Although only about 3 years have passed since the preparation of the original manuscript of this book for the Russian edition, the number of successful experiments on somatic hybridization of higher plants has doubled.

Author: Y. Y. Gleba

Publisher: Springer

ISBN: 3642822479

Category: Science

Page: 222

View: 490

Although only about 3 years have passed since the preparation of the original manuscript of this book for the Russian edition, the number of successful experiments on somatic hybridization of higher plants has doubled. Although the main inferences of the first edition still remain in force, most of them have received conclusive experimental support and, moreover, some new con clusions have been drawn. It can be expected that these inferences and conclusions will constitute a more or less durable foundation for somatic cell genetics of higher plants. We thus hope this book will also remain useful over the next years, in spite of the rapid progress of experiments and the increase in the number of scientific reports in this field. Though it might appear strange to an uninvolved observer, the principal progress in hybridization of somatic cells of higher plants has been due to plant physiologists (who entered the field by elaborating methods and techniques for plant cell and, later, for isolated protoplast culture) rather than plant geneticists. However, further qualitative improvement in this field is inconceivable without the instillation of genetic ideology and the strict logic of genetic experiments. The main purpose ofthis book is the attempt to organize the available experimental data in terms and cate gories of genetic analysis. For this reason, this book lays no claim on being a comprehensive treatise on somatic hybridization.
Categories: Science

Plant Protoplasts and Genetic Engineering V

Plant Protoplasts and Genetic Engineering V

Volumes already published Volume 1 : Trees I ( 1986 ) Volume 2 : Crops I ( 1986
) Volume 3 : Potato ( 1987 ) Volume 4 ... II ( 1988 ) Volume 7 : Medicinal and
Aromatic Plants II ( 1989 ) Volume 8 : Plant Protoplasts and Genetic Engineering I
 ...

Author: Professor Dr. Y. P. S. Bajaj

Publisher: Springer Science & Business Media

ISBN: 3540574476

Category: Technology & Engineering

Page: 414

View: 931

In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.
Categories: Technology & Engineering

Plant Protoplasts and Genetic Engineering

Plant Protoplasts and Genetic Engineering

Sequence of media for protoplast culture of Saintpaulia Day Medium ( see Table
4 ) Osmolarity PCM 560 mOsmol / kg H2O PCM CIM I 400 mOsmol / kg H20 CIM I
300 mOsmol / kg H2O CIM II CIM II CIM II CIM II dark at 26 °C for 15 h followed ...

Author: Y. P. S. Bajaj

Publisher:

ISBN: UOM:39015036077108

Category: Plant genetic engineering

Page:

View: 164

Categories: Plant genetic engineering

Somatic Embryogenesis and Synthetic Seed II

Somatic Embryogenesis and Synthetic Seed II

Volume 1 : Trees I ( 1986 ) Volume 2 : Crops I ( 1986 ) Volume 3 : Potato ( 1987 )
Volume 4 : Medicinal and Aromatic ... II ( 1988 ) Volume 7 : Medicinal and
Aromatic Plants II ( 1989 ) Volume 8 : Plant Protoplasts and Genetic Engineering I
 ...

Author: Y. P. S. Bajaj

Publisher: Springer

ISBN: WISC:89052268992

Category: Science

Page: 444

View: 471

While working in the laboratory of Professor Dr. Jacob Reinert at the Freie Universitat Berlin (1974-1976), I had the opportunity to become deeply involved in studying the intricacies of the fascinating phenomenon of somatic embryogenesis in plant cells and protoplasts. In numerous stimu lating discussions with Professor Reinert on this subject, I was fully convinced that somatic embryogenesis would become one of the most important areas of study, not only regarding basic and fundamental aspects, but also for its application in crop improvement. During the last decade, we have witnessed tremendous interest and achievements in the use of somatic embryos for the production of synthetic seeds, for micro prop a gation, genetic transformation, cryopreservation, and conservation of germplasm. The en masse production of somatic embryos in the bioreactors has facilitated some of these studies. Somatic embryos have now been induced in more than 300 plant species belonging to a wide range offamilies. It was therefore felt that a compilation ofliterature/state of the art on this subject was necessary. Thus, two volumes on Somatic Embryo genesis and Synthetic Seed have been compiled, which contain 65 chapters contributed by International experts. Somatic Embryogenesis and Synthetic Seed I comprises 31 chapters, arranged in 3 sections: Section I Commitment of the cell to somatic embryogenesis; early events; anatomy; molecular basis; gene expression; role of polyamines; machine vision analysis of somatic embryos. Section II Applications of somatic embryos; technology of synthetic seed; fluid drilling; micropropagation; genetic transfor mation through somatic embryos; cryopreservation.
Categories: Science