Oligonucleotide Synthesis

Oligonucleotide Synthesis

In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in ...

Author: Piet Herdewijn

Publisher: Springer Science & Business Media

ISBN: 9781592598236

Category: Science

Page: 435

View: 712

Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today.
Categories: Science

Oligonucleotide Synthesis

Oligonucleotide Synthesis

Oligonucleotide synthesis has become an essential technique required by many
biologically orientated laboratories . Yet up to now there has been no practical
manual available that adequately covers all the important techniques of synthetic
 ...

Author: Michael J. Gait

Publisher: Irl Press

ISBN: UOM:39015010046921

Category: Psychology

Page: 217

View: 804

Categories: Psychology

Biomedical Index to PHS supported Research

Biomedical Index to PHS supported Research

... OLIGONUCLEOTIDE SYNTHESIS FACILITY R43A1 - 35441 - 01
INTERCALATIVE ANTIGENE OLIGO ANALOGS AS ... 02 CHEMICAL
SYNTHESIS OF OLIGONUCLEOTIDE ANALOGUES 201BP - 02005 - 02
SUPPRESSION OF HIV - 1 IN ...

Author:

Publisher:

ISBN: MINN:31951P003778522

Category: Medicine

Page:

View: 567

Categories: Medicine

Cumulated Index Medicus

Cumulated Index Medicus

Intensification of genomic dactyloscopy by means of Methods Mol Biol 1993 ; 20 :
465-96 Biotechniques 1994 Apr ; 16 ( 4 ) : 702-7 hybridization with modified
oligonucleotides , forming Scale - up of oligonucleotide synthesis . Solution
phase .

Author:

Publisher:

ISBN: UIUC:30112023523431

Category: Medicine

Page:

View: 605

Categories: Medicine

Index Medicus

Index Medicus

OLIGONUCLEOTIDES CHEMICAL SYNTHESIS CPG oligonucleotides with
modified termini and nicked dumbbell Structure show enhanced ... Novel base-
labile protecting groups for 5'-hydroxy function in solidphase oligonucleotide
synthesis.

Author:

Publisher:

ISBN: OSU:32436011091848

Category: Medicine

Page:

View: 370

Categories: Medicine

Oligonucleotide Microarray Synthesis with a Micromirror Array

Oligonucleotide Microarray Synthesis with a Micromirror Array

New photolabile protecting groups in nucleoside and nucleotide chemistry -
Synthesis , cleavage mechanisms and applications . Nucleosides & Nucleotides
17 , 1987 - 1996 ( 1998 ) . 75 . Gao , X . L . et al . Oligonucleotide synthesis using
 ...

Author: Roland Daniel Green

Publisher:

ISBN: WISC:89065102790

Category:

Page: 282

View: 729

Categories:

Oligonucleotides as Therapeutic Agents

Oligonucleotides as Therapeutic Agents

The medicinal chemistry effort directed toward improving antisense and antigene
oligonucleotides has synthesized a large ... p 5-18 In recent years there has been
an explosion of efi'ort devoted to the synthesis of oligonucleotide analogues.

Author: Derek J. Chadwick

Publisher: John Wiley & Sons

ISBN: 9780470515402

Category: Science

Page: 260

View: 563

The use of oligonucleotides as therapeutic agents rests upon their ability to interfere, in a sequence-specific manner, with the fundamental machinery of protein synthesis either by binding to the mRNAs transcribed from a gene or by binding directly to a target gene. This approach can be used not only for inhibition of the synthesis of host proteins but also of those required by invading pathogens. Potential therapeutic applications are enormous, ranging over hypertension, cardiovascular disease, autoimmune disease, vital and other parasitic infections (especially HIV), and cancer. This book discusses the chemistry and pharmacokinetics of oligonucleotides and their analogues, and surveys the results of structure-activity studies and current clinical trials. It also critically reviews the problems with antisense therapy, such as the enzymatic destruction of oligonucleotides, the doses required for a therapeutic response, the difficulty in directing oligonucleotides to particular target tissues and cells, the need for parenteral administration, and doubts concerning the mechanism of action (especially problems associated with non-specific binding to proteins) and long-term effects.
Categories: Science

Synthesis and Applications of DNA and RNA

Synthesis and Applications of DNA and RNA

The savings get much more dramatic as the number of syntheses is increased.21
B. Linkage between the First Nucleoside/Nucleotide and the Polymer Support
Almost all polymer-supported oligonucleotide synthesis is directed from the 3' ...

Author: Narang Saran A

Publisher:

ISBN: 0125140304

Category: Science

Page: 237

View: 597

Synthesis And Applications Of DNA And RNA ...
Categories: Science

Comprehensive Natural Products Chemistry DNA and aspects of molecular biology

Comprehensive Natural Products Chemistry  DNA and aspects of molecular biology

... 390 phosphate hydrolysis , enzymatic , 372 phosphate mapping , studies , 270
phosphate protecting groups , in oligonucleotide synthesis , 130 phosphates ,
phenyl chloro- , as coupling reaction activator , 139 phosphinates , synthesis ,
297 ...

Author: Derek H. R. Barton

Publisher: Pergamon Press

ISBN: 0080431593

Category: Science

Page: 733

View: 734

Hardbound. This volume is intended to cover the chemistry of one of the most widely studied and important natural products, DNA. Discussed in detail are physicochemical properties of the molecule itself as well as small-molecule natural products that are known to interact with it. Also included are methods to synthesize and manipulate DNA and modified analogues. Twenty chapters are devoted to this overall topic.The first five relate to the structure of DNA; the first focuses on thermodynamics and kinetics of double helix formation; the next two describe triple- and tetra- helical structures formed by DNA; and the last two focus on methods for probing DNA structure (specifically, NMR methods and chemical probing methods, respectively).Chapters 6-12 focus on the chemistry of natural DNA and modified analogues. The first of these addresses nonenzymatic methods for synthesizing DNA, and the next chapter, on methods for attachment of reporter groups t
Categories: Science

Design Synthesis and Evaluation of Safety catch Photolabile Linkers

Design  Synthesis and Evaluation of Safety catch Photolabile Linkers

2 and any of the oligonucleotide synthesis reagents . This means that both the
dimethyl ketal and 1 , 3 - dioxolane could potentially be used as a safety - catch
in our linker design since they are stable to the conditions / reagents of
automated ...

Author: Shane T. Flickinger

Publisher:

ISBN: WISC:89094435229

Category:

Page: 146

View: 492

Categories:

Solid Supports and Catalysts in Organic Synthesis

Solid Supports and Catalysts in Organic Synthesis

2 APPLICATIONS OF OLIGONUCLEOTIDES The term oligonucleotide is often
used to mean specifically oligodeoxyribonucleotide , but this usage should be
avoided as methods for the synthesis of oligoribonucleotides are also now
available .

Author: K. Smith

Publisher: Ellis Horwood Limited

ISBN: UOM:39015028480674

Category: Catalyst supports.

Page: 338

View: 898

Categories: Catalyst supports.

The Application of Synthetically Modified Oligonucleotides to the Study of DNA Topoisomerase I

The Application of Synthetically Modified Oligonucleotides to the Study of DNA Topoisomerase I

While the use of 5 ' - phosphoramidites has been restricted primarily to the
synthesis of 5 ' - 5 ' and 3 ' - 3 ' linked oligonucleotides , ” cently efforts that exploit
automated DNA synthesis in a 5 ' → 3 ' direction have gained momentum . DNA
chip ...

Author: Christopher Dale Claeboe

Publisher:

ISBN: UVA:X004778478

Category:

Page: 388

View: 158

Categories:

Studies on the Functionalization of Synthetic Oligonucleotides on Solid support and Rolling Circle Replication Reactions

Studies on the Functionalization of Synthetic Oligonucleotides on Solid support and Rolling Circle Replication Reactions

6 On - column 5 ' - dabsylation of oligonucleotides – Results and Discussion 3 . 7
On - column 5 ' - dabsylation of oligonucleotides - Conclusions 3 . 8 Materials
and Methods 3 . 8 . 1 General method for oligonucleotide synthesis 3 . 8 .

Author: Gregory Powell Miller

Publisher:

ISBN: STANFORD:36105119732456

Category:

Page: 160

View: 577

Categories:

Indian Journal of Biochemistry and Biophysics

Indian Journal of Biochemistry and Biophysics

9) Oligonucleotide t-Butylphenoxyacetic anhydride NMI Scheme 4–
Oligonucleotide synthesis on a recyclable. DMTrC oco—-(E) Oligonucleotide
synthesis IM Spermin & 0.5 M LiClin aq. NH4OH HO-Oligomer-OH . __80° C. 10
min + Cyclic ...

Author:

Publisher:

ISBN: UIUC:30112048269952

Category: Biochemistry

Page:

View: 970

Categories: Biochemistry

The Journal of Biological Chemistry

The Journal of Biological Chemistry

Oligonucleotide synthesis v as low in dilute solutions of RNase A , rose steeply
as the enzyme concentration was increased , and reached a plateau at a
concentration of 40 ug per ml for Me - pUpU and pUpU . With U - cyclic - p the
plateau ...

Author:

Publisher:

ISBN: UOM:39015012035625

Category: Biochemistry

Page:

View: 176

Vols. 3-140 include the society's Proceedings, 1907-41
Categories: Biochemistry

Solid phase Synthesis

Solid phase Synthesis

26 Letsinger and Mahadevan : Oligonucleotide Synthesis on a Polymer Support
27 Letsinger and Mahadevan : Stepwise Synthesis of Oligodeoxyribonucleotides
on an Insoluble Polymer Support 28 Letsinger , Caruthers , and Jerina ...

Author: E. C. Blossey

Publisher:

ISBN: UOM:39015016095039

Category: Organic compounds

Page: 360

View: 522

Categories: Organic compounds

Gene Synthesis from Photolithographic DNA Microarrays

Gene Synthesis from Photolithographic DNA Microarrays

2 Chemical synthesis of oligonucleotides There are several methods of “ de novo
" oligonucleotide synthesis ; the dominant one is the phosphoramidite approach
developed by Caruthers and his coworkers in the early 1980s ( 4 , 5 , 6 ) .

Author: Changhan Kim

Publisher:

ISBN: WISC:89100033067

Category:

Page: 218

View: 947

Categories:

Innovation and Perspectives in Solid Phase Synthesis Combinatorial Libraries

Innovation and Perspectives in Solid Phase Synthesis   Combinatorial Libraries

28 OligoPrepTM : A New PVA Support for Oligonucleotide Synthesis at Large
Scale Zhiwei Wang , Isaiah Cedillo , Douglas L . Cole and Yogesh S . Sanghvi *
Development Chemistry , Isis Pharmaceuticals , Inc . , 2292 Faraday Avenue ...

Author: Roger Epton

Publisher:

ISBN: STANFORD:36105126892152

Category: Nucleic acids

Page: 343

View: 420

Categories: Nucleic acids

Russian Chemical Reviews

Russian Chemical Reviews

The third approach entails the synthesis of oligonucleotide conjugates in solution
. ... in the 2 - position should be stable under conditions of oligonucleotide
synthesis and be easily removed in the course of standard post - synthetic
treatments .

Author:

Publisher:

ISBN: STANFORD:36105121609718

Category: Chemistry

Page:

View: 454

Categories: Chemistry