Minton, A. P. (1994): Conservation of signal: a new algorithm for the elimination of reference concentration as an independent parameter in the analysis of sedimentation equilbrium data. In: Modern Analytical Ultracentrifugation: ...
Author: Todd M. Schuster
Publisher: Springer Science & Business Media
There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.
interferometer of the Optima XL-I AUC can be found in ref. 15. Besides these optical systems in modern AUCs, there is also the Schlieren optical system, which detects the refractive index/concentration gradient and belongs to the ...
Author: Stephen E Harding
Publisher: Royal Society of Chemistry
Analytical ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.
J Chem Phys 16:565 Kraemers EO, Nichols JB (1940) Results obtained with svedberg ultracentrifuges. ... Annu Rev Biophys Biomol Struct 28:75 Lebowitz J, Lewis MS, Schuck P (2002) Modern analytical ultracentrifugation in protein science: ...
Author: Susumu Uchiyama
This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC. This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included. The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis.
Modern applications of analytical ultracentrifugation. Annual Review of Biophysics and Biomolecular Structure, 28, 75–100. Lebowitz, J., Lewis, M. S., & Schuck, P. (2002). Modern analytical ultracentrifugation in protein science: A ...
Publisher: Academic Press
Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software. This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals. Timely contribution that describes a rapidly changing field Leading researchers in the field Broad coverage: instrumentation, basic theory, data analysis, and applications
3.5 ANALYTICAL CENTRIFUGATION 3.5.1 Applications of analytical ultracentrifugation As biological macromolecules exhibit random thermal motion, their relative ... 3.8 outlines the optical system of a modern analytical ultracentrifuge.
Author: Keith Wilson
Publisher: Cambridge University Press
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
A review with examples of advances in data acquisition and analysis in modern analytical centrifugation. Howlett, G.J., Minton, A.P. and Rivas, G. (2006) Analytical ultracentrifugation for the study of protein association and assembly.
Author: David Sheehan
Publisher: John Wiley & Sons
"As will be seen, there is not much missing here. I thought that the sections were well balanced, with rarely too much or too little on a given topic...This is a text to be welcomed by both teachers and students." BIOCHEMISTRY & MOLECULAR BIOLOGY EDUCATION (on the first edition) The second edition of this successful textbook explains the basic principles behind the key techniques currently used in the modern biochemical laboratory and describes the pros and cons of each technique and compares one to another. It is non-mathematical, comprehensive and approachable for students who are not physical chemists. A major update of this comprehensive, accessible introduction to physical biochemistry. Includes two new chapters on proteomics and bioinformatics. Introduces experimental approaches with a minimum of mathematics and numerous practical examples. Provides a bibliography at the end of each chapter. Written by an author with many years teaching and research experience, this text is a must-have for students of biochemistry, biophysics, molecular and life sciences and food science.
The temperature was measured in a very specific way: a needle was fixed to the bottom ofthe hanging analytical rotor, and a temperature-dependent ... Modern analytical ultracentrifuge Optima XL-A/I produced by Beckman-Coulter Fig. 2.3.
Author: Walter Maechtle
Publisher: Springer Science & Business Media
Category: Technology & Engineering
This book is divided into chapters covering instrumentation, sedimentation velocity runs, density gradient runs, application examples and future developments. In particular, the detailed application chapter demonstrates the versatility and power of AUC by means of many interesting and important industrial examples. Thus the book concentrates on practical aspects rather than details of centrifugation theory.
Because both components are highly sensitive to oxygen, the experiments were Schuster TM, Laue TM (eds) (1994) Modern analytical ultracentrifugation. Birkhauser,. Ó Springer-Verlag 1999 J. Behlke O. Ristau J. Behlke (8) á O. Ristau Max ...
Author: H. Cölfen
Category: Technology & Engineering
The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including: Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies. In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare. Therefore, this volume will be a helpful source of information to anyone who wants to catch up with the most recent developments and results related to this important analytical method.
Harding S, Horton J, Rowe A (eds) (1992) Analytical ultracentrifugation in biochemistry and polymer science. Royal Society of Chemistry, Cambridge 7. Schuster T and Laue T (eds) (1994) Modern analytical ultracentrifugation.
Author: W. Borchard
This volume includes 20 contributions of the 12th meeting on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. Various fields of ultracentrifugation are covered concerning research problems in biochemistry, biophysical chemistry and macromolecular chemistry as well as interacting systems. New investigations concerning the sedimentation theory are presented. The phase transition of gels is dealt with, as is the sedimentation-diffusion equilibrium of gels. One section contains the hydrodynamics of biopolymers.
... “On-line data acquisition for the Rayleigh interference optical system of the analytical ultracentrifuge,” in Modern Analytical Ultracentrifugation: Techniques and Methods, T.M. Schuster and T.M. Laue, Eds. Boston: Birkhäuser, 1994, ...
Author: Peter Schuck
Publisher: CRC Press
A follow-up to the experimental and instrumental aspects described in Basic Principles of Analytical Ultracentrifugation, the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles describes the theory and practice of data analysis. Mathematical models for the sedimentation process and the evolution of detected signals are developed in a comprehensive framework, jointly with the description of current and historical strategies for how to extract from noisy experimental data the physical parameters of interest, such as size, mass, and shape, composition, and polydispersity of sedimenting particles. The methods are extensively illustrated, and supported with practical applications, as well as cross-references where to find the methods in the public domain software SEDFIT and SEDPHAT. The systems covered are discrete or polydisperse mixtures of sedimenting molecules or particles in dilute solution, such as proteins and other biomolecules and their stable complexes, man-made polymers, and nanoparticles, observed in different optical systems. A useful reference for researchers and graduate students of macromolecular disciplines, these methods form the essential foundation for the analysis of dynamic interacting systems, which are covered in the volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems. Software referenced in the book is available for download at: https://sedfitsedphat.nibib.nih.gov/default.aspx