Imaging and Quantifying Neuronal Autophagy

Imaging and Quantifying Neuronal Autophagy

The chapters in this volume cover topics such as fundamental, historical, and functional approaches that began in baker’s yeast; Saccharomyces cerevisiae; the role of both electron microscopy and live-cell imaging using fluorescently ...

Author: Ben Loos

Publisher: Humana

ISBN: 1071615912

Category: Medical

Page: 0

View: 864

The volume aims to explore the dynamic nature of the autophagy pathway, and the latest techniques that allow researchers to capture and quantify this process in neurons. The chapters in this volume cover topics such as fundamental, historical, and functional approaches that began in baker’s yeast; Saccharomyces cerevisiae; the role of both electron microscopy and live-cell imaging using fluorescently tagged autophagy proteins; and the rate of puncta appearance and its correlation with the rate of autophagosome formation. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Imaging and Quantifying Neuronal is a valuable resource that provides insights into the power of microscopy tools, live-cell imaging, and photoactivation and correlative techniques.
Categories: Medical

Autophagy in Disease and Clinical Applications

Autophagy in Disease and Clinical Applications

Autophagic flux in axons and neurites is more difficult but may be possible using the image-based methods of sections 2.3.1–2.3.2 for quantifying GFP-LC3 puncta in the presence or absence of degradation inhibitors.

Author: Daniel Klionsky

Publisher: Academic Press

ISBN: 0080958486

Category: Science

Page: 536

View: 424

The third and final installment of Daniel J. Klionsky's new three-volume treatment of autophagy, this volume focuses on monitoring autophagy with regard to disease connections, and presents methods that can be used to analyze autophagy in clinical samples. Edited by one of the leading authorities in the field, this volume and its companion volumes, Autophagy: Lower Eukaryotes and Autophagy in Mammalian Systems, provide a comprehensive overview of the techniques involved in studying autophagy in eukaryotes and simple animal systems, mammalian cells and non-human animals, and humans. Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal “house cleaning in the cell is called autophagy – literally self-eating. In the future, research in this field will help to design clinical approaches that can turn on autophagy and halt tumor growth. * Provides an overview of autophagy in regards to humans, specifically regarding disease connections and clinical samples. * Includes methods to evaluate the role of autophagy in the drug-induced cell death of cancer cells in culture *Presents reliable methods that, in this relatively new field, allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes
Categories: Science

The Role of Neurovascular Unit in Neurodegeneration

The Role of Neurovascular Unit in Neurodegeneration

Digitally quantifying cerebral hemorrhage using photoshop and image J. J. Neurosci. ... a potential therapeutic mediator against Parkinson's disease via VEGF-induced angiogenesis and enhanced neuronal autophagy in neurovascular units.

Author: Dennis Qing Wang

Publisher: Frontiers Media SA

ISBN: 9782889761265

Category: Science

Page:

View: 355

Categories: Science

Psychiatric Disorders Late in Life

Psychiatric Disorders Late in Life

Loss of brain weight is reflected on neuroimaging as both ventricular enlargement, which can be multifactorial in etiology ... More sophisticated techniques for quantifying neurons have shown that the number of neurons in the cortex and ...

Author: Rajesh R. Tampi

Publisher: Springer

ISBN: 9783319730783

Category: Medical

Page: 416

View: 727

Though mental health recommendations for the elderly is rapidly evolving, the few current textbooks on this subject are either too voluminous or complex for regular review by clinicians, and most do not contain the latest information available in the field. Written by experts in geriatric psychiatry, this book provides a comprehensive yet concise review of the subject.The text covers topics that include the social aspect of aging, treatment and diagnosis options unique to the elderly in need of psychiatric care, policy and ethics, and particular geriatric health concerns that may influence psychiatric considerations. Psychiatric Disorders Late in Life is the ultimate resource for practicing psychiatrists, physicians, geriatricians, and medical students concerned with the mental healthcare of the elderly.
Categories: Medical

Autophagy in Autoimmunity

Autophagy in Autoimmunity

To extend our observation on macroautophagy and CMA of P140 in neuronal cells, we selected U-251MG ... These results were confirmed by measuring the expression levels of another macroautophagy-linked protein ...

Author: Xu-jie Zhou

Publisher: Frontiers Media SA

ISBN: 9782889458127

Category:

Page: 161

View: 352

Categories:

Molecular Characterization of Autophagic Responses Part B

Molecular Characterization of Autophagic Responses Part B

Image analysis procedures should be performed for green, red, and merge channels in all images (stacks). ... here to monitor autophagy activity in the central and peripheral nervous system is simple and allows measuring autophagy flux ...

Author:

Publisher: Academic Press

ISBN: 9780128097946

Category: Science

Page: 608

View: 254

Molecular Characterization of Autophagic Responses, Part B presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man. Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional. Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responses Written in an accessible style by renowned experts in the field
Categories: Science

Autophagy in Health and Disease

Autophagy in Health and Disease

Agents suitable for detecting autophagy by magnetic resonance imaging, positron emission tomography, or computed tomography ... proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects.

Author: Roberta A. Gottlieb

Publisher: Academic Press

ISBN: 9780123851017

Category: Medical

Page: 227

View: 333

Autophagy in Health and Disease offers an overview of the latest research in autophagy with a translational emphasis. This publication takes scientific research in autophagy a step further and offers integrated content with advancements in autophagy from cell biology and biochemical research to clinical treatments. A necessary reference for the bookshelf of medical and scientific researchers and students, Autophagy in Health and Disease presents high quality, reputable information on autophagy, allowing the reader quick access to the most applicable information. Discusses current understanding of the roles of autophagy in health and disease Covers the background of autophagy, the development of tools and therapeutics to measure and modulate autophagy, and autophagy in tissues and disease processes Features an accompanying website with figures and tables
Categories: Medical

Cell wide Metabolic Alterations Associated with Malignancy

Cell wide Metabolic Alterations Associated with Malignancy

Cellular imaging and analysis: For single-cell mitochondrial Ca2+ imaging, neurons transfected with ... and CFP and YFP emission were acquired by two HyDTM detectors for FRET ratio quantification at an acquisition rate of 1 frame/15 s.

Author:

Publisher: Academic Press

ISBN: 9780128014837

Category: Medical

Page: 384

View: 258

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods providing a a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo. Malignant cells exhibit metabolic changes when compared to their normal counterparts, owing to both genetic and epigenetic alterations. Although such a metabolic rewiring has recently been indicated as "yet another" general hallmark of cancer, accumulating evidence suggests that the metabolic alterations of each neoplasm rather represent a molecular signature that intimately accompanies, and hence cannot be severed from, all facets of malignant transformation. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics providing a a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo.
Categories: Medical

Factors Affecting Neurological Aging

Factors Affecting Neurological Aging

Transgenic expression of a ratiometric autophagy probe specifically in neurons enables the interrogation of brain autophagy in vivo. Autophagy, 15(3), 543e557. https://doi.org/10.1080/ 15548627.2018.1528812 Leidal, A. M., Levine, B., ...

Author: Colin R. Martin

Publisher: Academic Press

ISBN: 9780128179918

Category: Medical

Page: 686

View: 888

Factors Affecting Neurological Aging: Genetics, Neurology, Behavior, and Diet is a comprehensive reference on the genetic and behavioral features associated with neurological aging and associated disorders. This book discusses the mechanisms underlying neurological aging and provides readers with a detailed introduction to the aging of neural connections and complexities in biological circuitries, as well as the physiological, behavioral, molecular, and cellular features of neurological aging. Finally, this comprehensive resource examines the use of animal modeling of aging and neurological disease. Provides the most comprehensive coverage on a broad range of topics related to the neuroscience of aging Features sections on the genetic components that influence aging and diseases of aging Focuses on neurological diseases and conditions linked to aging, environmental factors and clinical recommendations Includes more than 500 illustrations and tables
Categories: Medical

mTOR in Human Diseases

mTOR in Human Diseases

These pieces of evidence corroborate findings showing that several autophagy inducers, such as lithium, rapamycin, and Food and Drug Administration (FDA) ... Notably, high-throughput image-based screens performed by Zhang et al.

Author: Olivier Dormond

Publisher: MDPI

ISBN: 9783039210602

Category: Medical

Page: 480

View: 499

The mechanistic target of rapamycin (mTOR) is a major signaling intermediary that coordinates favorable environmental conditions with cell growth. Indeed, as part of two functionally distinct protein complexes, named mTORC1 and mTORC2, mTOR regulates a variety of cellular processes, including protein, lipid, and nucleotide synthesis, as well as autophagy. Over the last two decades, major molecular advances have been made in mTOR signaling and have revealed the complexity of the events implicated in mTOR function and regulation. In parallel, the role of mTOR in diverse pathological conditions has also been identified, including in cancer, hamartoma, neurological, and metabolic diseases. Through a series of articles, this book focuses on the role played by mTOR in cellular processes, metabolism in particular, and highlights a panel of human diseases for which mTOR inhibition provides or might provide benefits. It also addresses future studies needed to further characterize the role of mTOR in selected disorders, which will help design novel therapeutic approaches. It is therefore intended for everyone who has an interest in mTOR biology and its application in human pathologies.
Categories: Medical