Principles of Enzyme Kinetics

Principles of Enzyme Kinetics

Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters.

Author: Athel Cornish-Bowden

Publisher: Elsevier

ISBN: 9781483164670

Category: Science

Page: 220

View: 636

Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.
Categories: Science

Fundamentals of Enzyme Kinetics

Fundamentals of Enzyme Kinetics

For the first time, this edition features a companion website providing all figures in colour www.wiley-vch.de/home/fundenzykinet

Author: Athel Cornish-Bowden

Publisher: John Wiley & Sons

ISBN: 9783527665495

Category: Science

Page: 510

View: 937

Now in its fourth edition, this textbook is one of the few titles worldwide to cover enzyme kinetics in its entire scope and the only one to include its implications for bioinformatics and systems biology. Multi-enzyme complexes and cooperativity are therefore treated in more detail than in any other textbook on the market. The respected and well known author is one of the most experienced researchers into the topic and writes with outstanding style and didactic clarity. As with the previous editions, he presents here steady-state kinetics and fast reactions, supplementing each chapter with problems and solutions. For the first time, this edition features a companion website providing all figures in colour www.wiley-vch.de/home/fundenzykinet
Categories: Science

Essential Enzyme Kinetics

Essential Enzyme Kinetics

Given the interdisciplinary nature of contemporary drug development, this book provides a lucid short-course that will also benefit nonspecialists seeking to understand the scope and reach of modern enzyme kinetics.

Author: Daniel L. Purich

Publisher: Academic Press

ISBN: 0128218088

Category: Science

Page: 425

View: 704

Essentials of Enzyme Kinetics: A Textbook for Molecular Life Scientists discusses best-practice approaches for using site-directed mutagenesis to explore enzyme catalysis. The book presents a balance of initial-rate methods, fast reaction techniques, and kinetic isotope effect approaches, providing a must-have reference for bioscience workers and investigators who seek a better understanding of enzyme kinetics. Non-biochemists entering careers in Big Pharma will also benefit from the book's content as covered topics have become main drivers in small-molecule drug development. Offers practical information on how to work with enzymes and design experiments to identify new inhibitors or activators Provides detailed, step-by-step derivations of rate equations, showing tried-and-true ways to confirm that equations are correct Includes 10-12 problems for each chapter
Categories: Science

Enzyme Kinetics

Enzyme Kinetics

This series of books is designed to satisfy this need. The authors have been asked to produce abrief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology.

Author: Paul C. Engel

Publisher: Springer

ISBN: UOM:39015009028864

Category: Science

Page: 96

View: 830

The student of biological science in his final years as an undergraduate and his first years as a graduate is expected to gain some familiarity with current research at the fron tiers of his discipline. New research work is published in a perplexing diversity of publications and is inevitably concerned with the minutiae of the subject. The sheer number of research journals and papers also causes confusion and difficulties of assimilation. Review articles usually presuppose a background knowledge of the field and are inevitably rather restricted in scope. There is thus a need for short but authoritative introductions to those areas of modern biological research which are either not dealt with in standard introductory text books or are not dealt with in sufficient detail to enable the student to go on from them to read scholarly reviews with profit. This series of books is designed to satisfy this need. The authors have been asked to produce abrief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology.
Categories: Science

Kinetics of Enzyme Modifier Interactions

Kinetics of Enzyme Modifier Interactions

This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions.

Author: Antonio Baici

Publisher: Springer

ISBN: 9783709114025

Category: Science

Page: 489

View: 760

The kinetic mechanisms by which enzymes interact with inhibitors and activators, collectively called modifiers, are scrutinized and ranked taxonomically into autonomous species in a way similar to that used in the biological classification of plants and animals. The systematization of the mechanisms is based on two fundamental characters: the allosteric linkage between substrate and modifier and the factor by which a modifier affects the catalytic constant of the enzyme. Combinations of the physically significant states of these two characters in an ancestor-descendant-like fashion reveal the existence of seventeen modes of interaction that cover the needs of total, partial and fine-tuning modulation of enzyme activity. These interactions comprise five linear and five hyperbolic inhibition mechanisms, five nonessential activation mechanisms and two hybrid species that manifest either hyperbolic inhibition or nonessential activation characteristics depending on substrate concentration. Five essential activation mechanisms, which are taxonomically independent of the mentioned basic species, complete the inventory of enzyme modifiers. Often masked under conventional umbrella terms or treated as anomalous cases, all seventeen basic inhibition and nonessential activation mechanisms are represented in the biochemical and pharmacological literature of this and the past century, either in the form of rapid or slow-onset reversible interactions, or as irreversible modification processes. The full potential of enzyme inhibitors and activators can only be appreciated after elucidating the details of their kinetic mechanisms of action exploring the entire range of physiologically significant reactant concentrations. This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions. The reader is guided in the journey through this still partly uncharted territory with the aid of mechanistically-oriented criteria aimed at showing the logical way towards the identification of a particular mechanism.
Categories: Science

Enzyme Kinetics Catalysis and Control

Enzyme Kinetics  Catalysis and Control

Haldane Relations can be Used to Distinguish Kinetic Mechanisms of Bisubstrate Enzyme-Catalyzed Reactions The Dalziel ... Some Reversible Essential Activators Bind After the Substrate Some Enzymes Randomly Bind Essential Activators and ...

Author: Daniel L. Purich

Publisher: Elsevier

ISBN: 9780123809254

Category: Science

Page: 920

View: 170

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions
Categories: Science

Enzyme Kinetics and Mechanisms

Enzyme Kinetics and Mechanisms

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments.

Author: Kenneth B. Taylor

Publisher: Springer Science & Business Media

ISBN: 9781402007286

Category: Science

Page: 227

View: 461

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature. Enzyme Kinetics and Mechanisms is intended for research workers, graduate students, post doctoral associates, and faculty in biochemistry and molecular biology who are interested in the techniques and logic by which mechanisms of enzymes-catalyzed reactions can be elucidated by investigation of steady-state kinetic experiments.
Categories: Science

Enzyme Kinetics

Enzyme Kinetics

Substrate pK's may of course be measured separately without reference to the enzyme-catalysed reaction. ... 4.5 Ionization of 'essential' residues Within the structure of an enzyme molecule, some residues play a more crucial role than ...

Author: Paul C. Engel

Publisher: Springer

ISBN: 9781489932785

Category: Science

Page: 96

View: 157

The student of biological science in his final years as an undergraduate and his first years as a graduate is expected to gain some familiarity with current research at the fron tiers of his discipline. New research work is published in a perplexing diversity of publications and is inevitably concerned with the minutiae of the subject. The sheer number of research journals and papers also causes confusion and difficulties of assimilation. Review articles usually presuppose a background knowledge of the field and are inevitably rather restricted in scope. There is thus a need for short but authoritative introductions to those areas of modern biological research which are either not dealt with in standard introductory text books or are not dealt with in sufficient detail to enable the student to go on from them to read scholarly reviews with profit. This series of books is designed to satisfy this need. The authors have been asked to produce abrief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology.
Categories: Science

Enzyme Kinetics and Mechanisms

Enzyme Kinetics and Mechanisms

Some understanding of calculus is necessary to understand the curve-fitting algorithms and the slow inhibitors, but these are not essential for the understanding of steady-state enzyme kinetics. An understanding of the material in this ...

Author: Kenneth B. Taylor

Publisher: Springer Science & Business Media

ISBN: 9780306480256

Category: Science

Page: 228

View: 676

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature.
Categories: Science