The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes.
Author: Chunhai Fan
Publisher: Springer Nature
Category: Technology & Engineering
The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. The chapter "DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials" is available open access under a CC BY 4.0 License via link.springer.com.
This second edition provides new and updated methods that detail new DNA nanotechnology techniques.
Author: Giampaolo Zuccheri
This second edition provides new and updated methods that detail new DNA nanotechnology techniques. Chapters focus on DNA origami nanostructures for arranging matter in the nanoscale or on their manipulation with the aid of other technologies, on procedures for making nucleic acids nanostructures of different kinds, and methods to simulate complex nanostructures or to use them in biosensing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Nanotechnology: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Author: Arrabito Giuseppe DomenicoPublish On: 2017-09-25
This book is intended for non-specialists and students, presenting a unique introduction to the field of DNA nanotechnology.
Author: Arrabito Giuseppe Domenico
Publisher: World Scientific
Category: Technology & Engineering
This book is intended for non-specialists and students, presenting a unique introduction to the field of DNA nanotechnology. The primary focus is on the extraordinary advantages of specificity and sensitivity obtained by integrating DNA nanostructures in bioanalytical devices. DNA Nanotechnology for Bioanalysis provides a concise and rigorous description for the fabrication of various types of functional nanostructures by optimized software-aided high-yield synthesis. Following this is the explanation of methods to decorate these nanostructures with molecules such as proteins, metal nanoparticles or bioorganic moieties covalently bonded onto DNA via self-assemblage processes. Also provided is a concise review on non-canonical DNA structures (such as G-quadruplexes) and their targeting by small molecules for applications in pharmacology. Finally, it describes the exciting applications of DNA nanostructures in life sciences and nanomedicine, including ultraspecific molecular delivery, control of cell behavior, analysis of cell lysate and DNA-based nano-tools for super-resolution sub-cellular imaging. Contents: Nanotechnology and the Unique Role of DNA (Elisa-C Schöneweiβ, Andreas Jaekel and Barbara Saccà)DNA as Building Material at the Nanoscale: From Concepts to Software-aided Design (Wolfgang Pfeifer, Georg Homa, Giuseppe Arrabito and Barbara Saccà)DNA Sensors for the Detection of Biomolecules and Biochemical Conditions (Birgitta R Knudsen, Anni H Andersen, Magnus Stougaard, Giuseppe Arrabito, Raffaella Suriano and Yi-Ping Ho)DNA Nanostructures in Cell Biology and Medicine (Liqian Wang and Giuseppe Arrabito)Targeting G-quadruplex DNA as Potential Anti-cancer Therapy (Riccardo Bonsignore, Elisa Trippodo and Giampaolo Barone)DNA-aided Super-resolution Bioimaging (JiaJun Li, Giuseppe Arrabito and ZhaoShuai Gao) Readership: Non-specialists, undergraduate students and researchers who are interested in learning the basics of DNA nanotechnology. Keywords: DNA Nanotechnology;Nanostructures;Non-Canonical DNA Structures;G Quadruplexes;Nano-Tools;DNA Origami;DNA Aptamers;Biosensors;Drug DeliveryReview: Key Features: Constitutes a clear introduction to the field of DNA NanotechnologyWritten in an easy and catchy styleContains information of the most recent findings in the world of DNA Nanotechnology (up to 2016), optimal for researchers who want to keep updated with the latest breakthroughs in the field
Author: Thimmaiah GovindarajuPublish On: 2019-01-28
This book can serve as a practical handbook or as a textbook for advanced undergraduate- and graduate-level students of nanotechnology and DNA nanotechnology, supramolecular chemistry, and nanoarchitectonics and researchers working on ...
Author: Thimmaiah Govindaraju
Publisher: CRC Press
Category: Technology & Engineering
Nucleic acids have structurally evolved over billions of years to effectively store and transfer genetic information. In the 1980s, Nadrian Seeman’s idea of constructing a 3D lattice from DNA led to utilizing DNA as nanomolecular building blocks to create emergent molecular systems and nanomaterial objects. This bottom-up approach to construct nanoscale architectures with DNA marked the beginning of a new field, DNA nanotechnology, contributing significantly to the broad area of nanoscience and nanotechnology. The molecular architectonics of small "designer" molecules and short DNA sequences through complementary binding interaction engenders well-defined functional nanoarchitectures with realistic applications in areas ranging from biology to materials science and is termed "DNA nanoarchitectonics." This book discusses novel approaches adapted by leading researchers from all over the world to create functional nucleic acid molecular systems and nanoarchitectures. Individual chapters contributed by active practitioners provide fundamental and advanced knowledge emanated from their own and others’ work. Each chapter includes numerous illustrations, historical perspectives, case studies and practical examples, critical discussions, and future prospects. This book can serve as a practical handbook or as a textbook for advanced undergraduate- and graduate-level students of nanotechnology and DNA nanotechnology, supramolecular chemistry, and nanoarchitectonics and researchers working on macromolecular science, nanotechnology, chemistry, biology, and medicine, especially those with an interest in sensors, biosensors, nanoswitches and nanodevices, diagnostics, drug delivery, and therapeutics.
This is interesting for sensing applications, especially when the analyte produces large scale, detectable structural changes, and also for creating molecular muscles, capable of extension and contraction under external control.
Author: Amani Hariri
"By taking it out of its biological role, DNA has served as a robust, reliable and programmable nanofabrication building block for the assembly of a variety of two- and three-dimensional discrete nanometer-sized structures with arbitrary shapes and designs. These molecularly addressable materials could serve as platforms for the site-specific hybridization with nanometer precision. Besides structural complexity, DNA objects evolved to comprise an intrinsic dynamic character allowing them to respond to surrounding stimuli in a predictable manner. Many researchers have previously constructed nanostructures using methods that rely on the spontaneous assembly of DNA in solution, in which case the more complex the assembly design is, the more error-prone the product becomes. This constitutes a major problem for the application of DNA-based structures in dynamic devices such as machines, motors, robots, and computers, where even small errors in assembly can drastically affect performance. Therefore, quantitative tools for analysing structures and dynamics of complex DNA nanostructures are critical. Single molecule fluorescence techniques have been transformative to the field of nanoscience and have played a leading role in providing a mature understanding and an informative feedback on the building blocks and final products of self-assembly. This thesis demonstrates how different single molecule fluorescence methodologies can be utilized to study and advance the structure, dynamics and integrity of DNA-assemblies, specifically those shaped as nanotubes. Conceptually, this research can be divided into four main parts: (i) synthesis optimization, (ii) structural characterization, (iii) monitoring dynamic structures, and (iv) studying structural dynamics. First, a solid-phase synthesis strategy and its visualization through single-molecule spectroscopy was devised to assemble DNA nanotubes in a stepwise fashion, with a full control over their size and sequence pattern. This method paves the way for the production of custom-made DNA nanotubes with fewer structural flaws than the spontaneous-assembly method. Second, single molecule photobleaching and two-color colocalization approaches were uniquely combined to provide a systematic way of assessing the polydispersity, stoichiometry and degree of defectiveness of different DNA nanotubes structures. These approaches will be of significant importance for many research groups synthesizing large supramolecular structures or studying naturally occurring ones, such as protein clusters, amyloids, etc. Third, in situ single molecule immobilization-based fluorescence microscopy was employed to introduce structural changes into DNA nanotubes by dynamically adjusting one or several of the edge lengths between the building blocks using a combination of strand displacement and loops. This is interesting for sensing applications, especially when the analyte produces large scale, detectable structural changes, and also for creating molecular muscles, capable of extension and contraction under external control. Lastly, dynamics and robustness of DNA nanotubes, reconfigured in response to site-specific deletion of DNA strands, were investigated using two color single molecule microscopy. This strategy enables to develop a better understanding of the collective structural changes within DNA structures in response to modifications in their repeat unit. Together, the different methods developed in this thesis underline the importance of single molecule techniques as powerful tools which can advance the field of DNA nanotechnology by enabling the production of well-defined high-quality objects that can meet the designer's compositional and dynamic specifications." --
DNA - based nanomechanical devices can lead to a nanometer - scale robotics
and to very smart materials , materials that respond to specific stimuli by
particular spatial transitions . Structural DNA nanotechnology creates motifs that
can be ...
This work examines the dynamic addressability of the cages on bilayers, their diffusion properties and depth of anchored cages, and the bilayer templated assembly of DNA structures.
Author: Justin Conway
"Deoxyribonucleic acid (DNA) has evolved in nature to store and transfer the genetic information of all life on earth. The fidelity of information processing relies on the precise pairing through non-covalent interactions of a molecular code consisting of four unique nucleobases. By using this programmability DNA can be taken of a biological context and used as building material for the programmable assembly of nanostructures. The routine automated synthesis of DNA has allowed researchers to explore many different DNA architecture designs and applications leading to the creation of the diverse field now termed DNA nanotechnology. DNA nanotechnology has generated many examples of scaffolds, cages, and networks able to precisely position molecules for applications in therapeutics, diagnostics, light harvesting devices, nanopatterning and even molecular computing. The objective of this thesis is to expand the interface between DNA nanotechnology and biological systems. In this way, DNA nanostructures can be used as modular platforms for the design of cellular probes and drug delivery vehicles. We focus our efforts on examining DNA and lipid bilayer interactions as well as increasing DNA nanostructure serum stability.Chapter 1 of this thesis discusses the origins and evolution of the field of DNA nanotechnology and focuses on specific examples within all three major divisions of this field. Chapter 2 describes our work investigating spherically supported bilayers as a platform to land and reversibly assemble DNA cages. This work examines the dynamic addressability of the cages on bilayers, their diffusion properties and depth of anchored cages, and the bilayer templated assembly of DNA structures. Chapter 3 investigates the selective deposition of DNA tile networks on saturated and unsaturated supported lipid bilayers using three structurally different hydrophobic anchors. It is shown that correct network assembly only occurs with compatible packing between the DNA-anchor and the lipid alkyl chains. The variation of the anchor and bilayer chemistry generates switchable network morphologies and filamentous materials. Chapter 4 describes our research towards improving the serum stability of DNA nanostructures, using a combination of folding topology and small synthetic end modifications to the DNA. This work uses a DNA triangular prism cage formed from three DNA strands. It is shown that the folded architecture of these cages and small chemical modifications on the DNA ends significantly stabilize the single stranded DNA and the final cages from fetal bovine serum degradation. We also show how this cage can be fully ligated to create a closed structure with the highest observed serum stability. These projects intend to demonstrate new ways in which DNA nanotechnology can be applied to biological systems for both medicinal and material based applications." --
 Q. Hu, H. Li, L. Wang, H. Gu, C.H. Fan, DNA nanotechnology-enabled drug
delivery systems. Chem. Rev. (2018). ...  R.P. Goodman, R.M. Berrya, A.J.
Turberfielda, The single-step synthesis of a DNA tetrahedron, Chem. Commun.
Publisher: Academic Press
Advances in Clinical Chemistry, Volume 91, the latest release in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians and research scientists. The serial discusses the latest and most up-to-date technologies related to the field of clinical chemistry, providing the benchmark for novel analytical approaches in the clinical laboratory. Provides the most up-to-date technologies in clinical chemistry and clinical laboratory science Authored by world renowned clinical laboratory scientists, physicians and research scientists Presents the international benchmark for novel analytical approaches in the clinical laboratory
Bio - Nanotechnology Laboratory , Research Institute for Electronic Science ,
Hokkaido University , Sapporo 060 - 0812 , Japan RESEARCH ARTICLE
Liverwort - like DNA microscale structures consist of 4 - sticky - end Holiday
junctions as ...
It is useful to distinguish structural DNA nanotechnology from a less precise form
of DNA nanotechnology , termed compositional DNA nanotechnology . Structural DNA nanotechnology uses well structured components , combined by using ...
This book covers the emerging topic of DNA nanotechnology and DNA supramolecular chemistry in its broader sense.
Author: Eugen Stulz
Publisher: John Wiley & Sons
This book covers the emerging topic of DNA nanotechnology and DNA supramolecular chemistry in its broader sense. By taking DNA out of its biological role, this biomolecule has become a very versatile building block in materials chemistry, supramolecular chemistry and bio-nanotechnology. Many novel structures have been realized in the past decade, which are now being used to create molecular machines, drug delivery systems, diagnosis platforms or potential electronic devices. The book combines many aspects of DNA nanotechnology, including formation of functional structures based on covalent and non-covalent systems, DNA origami, DNA based switches, DNA machines, and alternative structures and templates. This broad coverage is very appealing since it combines both the synthesis of modified DNA as well as designer concepts to successfully plan and make DNA nanostructures. Contributing authors have provided first a general introduction for the non-specialist reader, followed by a more in-depth analysis and presentation of their topic. In this way the book is attractive and useful for both the non-specialist who would like to have an overview of the topic, as well as the specialist reader who requires more information and inspiration to foster their own research.
The other story looks back 4 billion years to the possible origins of DNA which are shrouded in mystery. The book is divided into three parts comprised of 15 chapters and two Brief Interludes.
Author: Kenneth Douglas
Publisher: CRC Press
DNA Nanoscience: From Prebiotic Origins to Emerging Nanotechnology melds two tales of DNA. One is a look at the first 35 years of DNA nanotechnology to better appreciate what lies ahead in this emerging field. The other story looks back 4 billion years to the possible origins of DNA which are shrouded in mystery. The book is divided into three parts comprised of 15 chapters and two Brief Interludes. Part I includes subjects underpinning the book such as a primer on DNA, the broader discipline of nanoscience, and experimental tools used by the principals in the narrative. Part II examines the field of structural DNA nanotechnology, founded by biochemist/crystallographer Nadrian Seeman, that uses DNA as a construction material for nanoscale structures and devices, rather than as a genetic material. Part III looks at the work of physicists Noel Clark and Tommaso Bellini who found that short DNA (nanoDNA) forms liquid crystals that act as a structural gatekeeper, orchestrating a series of self-assembly processes using nanoDNA. This led to an explanation of the polymeric structure of DNA and of how life may have emerged from the prebiotic clutter.
These properties together endow DNA molecules with a great potential to serve
as “ building blocks ” for the preparation of nanostructures . In structural DNA nanotechnology , DNA motifs ( tiles ) are designed and investigated as building ...
Publisher: Faraday Discussions
This book will incorporate aspects of structuring soft-materials at the nanoscale and the incorporation of such materials into actual devices. Soft nanotechnology aims to build on our knowledge of biological systems, by implementing self-assembly and 'wet chemistry' into electronic devices, actuators, fluidics, etc. Understanding, predicting and utilising the rules of self-assembly (be it at solid liquid interfaces, in solution, or in block copolymers) and interface the resulting complex structures in well-defined 2D and 3D arrangements. This timely book will appeal to scientists, researchers and anyone working in this field.
INTRODUCTION DNA Nanotechnology DNA nanotechnology can be divided into
two parts based on the length of the applied molecules : The oligonucleotide -
based part includes DNA chip technology and the bioconjugation of inorganic ...
Author: Wolfgang Fritzsche
Publisher: American Institute of Physics
The conference focuses on the various applications of DNA for future molecular electronics. The main topics are the characterization of DNA conductivity, modification of DNA in order to generate biotemplated nanowires, and the use of DNA to connect or position other nanostructures such as carbon nanotubes.
As pointed out earlier , DNA molecules not only are stable , three - dimensional
structures that function efficiently as nanomachines in living cells , but also they
selfreplicate naturally and spontaneously . Some researchers are exploring ways
Author: David E. Newton
Category: Technology & Engineering
This book covers an exciting new field involving the manipulation of individual atoms and molecules to produce materials and devices with very precise, predictable properties.