Culture of Animal Cells

A Manual of Basic Technique and Specialized Applications

Author: R. Ian Freshney

Publisher: John Wiley & Sons

ISBN: 1118873378

Category: Science

Page: 728

View: 3571

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Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
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Culture of Animal Cells

A Manual of Basic Technique and Specialized Applications

Author: R. Ian Freshney

Publisher: John Wiley & Sons

ISBN: 9780470649350

Category: Science

Page: 768

View: 7703

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This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture
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Studyguide for Culture of Animal Cells

A Manual of Basic Technique and Specialized Applications by Freshney, R. Ian

Author: Cram101 Textbook Reviews,Cram101 Textbook Reviews Staff

Publisher: Cram101

ISBN: 9781478475804

Category:

Page: 224

View: 670

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Never HIGHLIGHT a Book Again Includes all testable terms, concepts, persons, places, and events. Cram101 Just the FACTS101 studyguides gives all of the outlines, highlights, and quizzes for your textbook with optional online comprehensive practice tests. Only Cram101 is Textbook Specific. Accompanies: 9780872893795. This item is printed on demand.
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Culture of Human Tumor Cells

Author: Roswitha Pfragner,R. Ian Freshney

Publisher: John Wiley & Sons

ISBN: 0471270628

Category: Science

Page: 452

View: 8373

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This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed. An extensive listing of vendors for equipment and other cell culture products is also included.
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Animal Biotechnology

Models in Discovery and Translation

Author: Ashish Verma,Anchal Singh

Publisher: Academic Press

ISBN: 0123914345

Category: Science

Page: 668

View: 4642

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Animal Biotechnology introduces applications of animal biotechnology and implications for human health and welfare. It begins with an introduction to animal cell cultures and genome sequencing analysis and provides readers with a review of available cell and molecular tools. Topics here include the use of transgenic animal models, tissue engineering, nanobiotechnology, and proteomics. The book then delivers in-depth examples of applications in human health and prospects for the future, including cytogenetics and molecular genetics, xenografts, and treatment of HIV and cancers. All this is complemented by a discussion of the ethical and safety considerations in the field. Animal biotechnology is a broad field encompassing the polarities of fundamental and applied research, including molecular modeling, gene manipulation, development of diagnostics and vaccines, and manipulation of tissue. Given the tools that are currently available and the translational potential for these studies, animal biotechnology has become one of the most essential subjects for those studying life sciences. Highlights the latest biomedical applications of genetically modified and cloned animals with a focus on cancer and infectious diseases Provides firsthand accounts of the use of biotechnology tools, including molecular markers, stem cells, and tissue engineering
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Atlas of Living Cell Cultures

Author: Toni Lindl,Rosemarie Steubing

Publisher: John Wiley & Sons

ISBN: 3527669930

Category: Science

Page: 521

View: 981

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The first atlas in many years giving researchers a good visual reference of the status of their cell lines. Given the increasing importance of well defined cellular models in particular in biomedical research this is a sorely needed resource for everyone performing cell culture.
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Biotechnology

An Introduction

Author: Maria Pele,Carmen Cimpeanu

Publisher: WIT Press

ISBN: 1845646665

Category: Medical

Page: 315

View: 4093

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Comprising seven chapters, this book comprehensively covers all topics of biotechnology. A unique, concise and up-to-date resource, it offers readers an innovative and valuable presentation of the subject. It has been carefully prepared to present the concepts with the help of diagrams, figures and tables. It covers the fundamental aspects and applications of biotechnology for the production of valuable products adn services. Each chapter is presented in a simple and systematic way to provide a thorough understanding of the core principles of science, the interrelationships between biotechnology of the core principles of science, the interrelationships between biotechnology and other disciplines and how biotechnology affects our everyday lives. The basicconcepts of each step to be followed in developing a biotechnology process are clearly explained and their functions are highlighted. Recent developments in other fields have also been included to provide a contemporary understanding of the subject and the large domain of biotechnology applications. The last chapter contains some of the most recent examples of biotechnology applications such as green chemistry or environmental biotechnology. Finally the book presents an annex which contains some of the most important discoveries that led to the development of biotechnology today.
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Pflanzenernährung

Author: D. I. Arnon,W. Baumeister,W. U. Behrens,E. v. Boguslawski,M. J. Bukovac,H. Burghardt,M. Erichsen,W. Flaig,W. H. Fuchs,F. Grossmann,F. Heilinger,G. P. Hill,J. Jung,H. Kick,J. G. Kisser,P. J. Kramer,H. Kühn,P. Limberg,H. Linser,H. H. Mayr,K. Mengel,K.-H. Neumann,K. Scharrer,G. Schmid,W. Schuster,H. B. Tukey,H. Walter,S. H. Wittwer

Publisher: Springer-Verlag

ISBN: 3709182204

Category: Science

Page: 594

View: 2379

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Seit dem Erscheinen des zweibändigen Standardwerkes von F. HoNCAMP, "Handbuch der Pflanzenernährung und Düngerlehre", sind mehr als dreißig Jahre vergangen. Bei den stürmischen Fortschritten auf allen Gebieten der Natur wissenschaften, insbesondere der Chemie, Pflanzenphysiologie, Biochemie, Bodenkunde und Technologie, ist dieses zur Zeit seines Erscheinens so aus gezeichnete Handbuch natürlich längst veraltet. Zweifellos besteht heute ein dringendes Bedürfnis nach einem ähnlichen Werk. Wenn wir von dem ursprünglichen Plan abgekommen sind, das HoNCAMP sche Handbuch in neuer Auflage zu bearbeiten, so vor allem deswegen, weil die tiefgreifende Entwicklung auf diesem Gebiet eine völlig neue Gestaltung des Werkes geboten erscheinen ließ. Es gliedert sich nun in drei Bände. Band I behandelt im wesentlichen die physiologischen Grundlagen der Pflanzenernährung. Er enthält eine ausführliche und systematische Darstellung der eigentlichen Ernährungsphysiologie. Auf eine Erörterung der allgemeinen pflanzenphysiologischen Grundlagen konnte dabei verzichtet werden, weil im RuHLANDschEin "Handbuch der Pflanzenphysiologie" ein umfassendes und modernes Nachschlagewerk zur Verfügung steht. Band II beschäftigt sich mit dem Boden und den Düngemitteln. Unter Berücksichtigung der modernen Agrikulturchemie haben wir das heutige Wissen über den Boden als Standort und Nährstoffquelle der Pflanze unter den Verhält nissen der landwirtschaftlichen Praxis und des Gartenbaues bzw. des Forstwesens systematisch zusammengefaßt. Die folgenden Kapitel befassen sich mit der Beschreibung und Herstellung der verschiedenen Wirtschafts- und Handels düngemittel. Gerade auf diesem Gebiet bestehen in der wissenschaftlichen Literatur erhebliche Lücken, da keine größeren Lehr- oder Handbücher vorhanden sind, die sich eingehender mit der Technologie der Düngemittel und der Düngung beschäftigen.
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Tissue Culture Techniques

An Introduction

Author: Bernice M. Martin

Publisher: Springer Science & Business Media

ISBN: 9780817636432

Category: Medical

Page: 247

View: 9202

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ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations • Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat • Radiation • Toxic gas • Filtration • Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions • water as a reagent· Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells • Common enzyme solutions • Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer • Particle counter • Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi • Fungi • Mycoplasma • Viruses • Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth • Recurrent contamination • When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards • Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum • Treatments of serum • Plasma-derived serum • Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 Coatingplasticware with solutions • Alterations with polymers • Using cells to coat the plasticware • Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes • Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection • Enzymatic dissociation methods • Nonenzymatic isolation • Purification of cell suspensions • Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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