So here is where the new paradigm of biofilm infection meets the equally new paradigm of the culture negativity of biofilms, and this volume presents a conceptual synthesis that may soon combine the most effective molecular methods for the ...
Author: Garth D. Ehrlich
Publisher: Springer Science & Business Media
During the recent transition between acute diseases caused by swarms of single planktonic bacteria, and chronic infections caused by bacteria growing in slime-enclosed biofilms, a general clinical consensus has emerged that pathologies with bacterial etiologies are frequently culture negative. Because biofilm infections now affect 17 million Americans per year (killing approximately 450,000), the suggestion that these common and lethal infections regularly go unnoticed by the only FDA-approved method for their detection and characterization is a matter of urgent concern. Biologically, we would expect that planktonic bacterial cells would colonize any new surface, including the surface of an agar plate, while the specialized sessile cells of a biofilm community would have no such proclivity. In the study of biofilm diseases ranging from otitis media to prostatitis, it was found that direct microscopy and DNA- and RNA-based molecular methods regularly document the presence of living bacteria in tissues and samples that are culture negative. The editors selected orthopedic biofilm infections as the subject of this book because these infections occur against a background of microbiological sterility in which modern molecular methods would be expected to find bacterial DNA, RNA-based microscopic methods would be expected to locate bacterial cells, and cultures would be negative. Moreover, in Orthopedics we find an already biofilm-adapted surgical group in which current strategies are based on the meticulous removal of compromised tissues, antibiotic options as based on high biofilm-killing local doses, and there are practical bedside strategies for dealing with biofilm infections. So here is where the new paradigm of biofilm infection meets the equally new paradigm of the culture negativity of biofilms, and this volume presents a conceptual synthesis that may soon combine the most effective molecular methods for the detection and identification of bacteria with a surgical discipline that is ready to help patients.
Int J Artif Organs 28:1091–1100 Arciola CR, Baldassarri L, Campoccia D et al (
2008) Strong biofilm production, antibiotic ... Ehrlich GD, DeMeo P, Costerton JW,
Winkler H (eds) (2012) Culture-negative orthopedic biofilm infections. Springer ...
Author: Gianfranco Donelli
The aim of this book is to provide readers with a wide overview of the main healthcare-associated infections caused by bacteria and fungi able to grow as biofilm. The recently acquired knowledge on the pivotal role played by biofilm-growing microorganisms in healthcare-related infections has given a new dynamic to detection, prevention and treatment of these infections in patients admitted to both acute care hospitals and long-term care facilities. Clinicians, hygienists and microbiologists will be updated by leading scientists on the state-of-art of biofilm-based infections and on the most innovative strategies for prevention and treatment of these infections, often caused by emerging multidrug-resistant biofilm-growing microorganisms.
the fact that the biofilm phenotype differs so profoundly from the planktonic
phenotype of the same strain, any attempts to ... The problem of overt culture- negative infections has bedeviled clinicians in orthopedic surgery, ear nose and
Publisher: Academic Press
Available as an exclusive product with a limited print run, Encyclopedia of Microbiology, 3e, is a comprehensive survey of microbiology, edited by world-class researchers. Each article is written by an expert in that specific domain and includes a glossary, list of abbreviations, defining statement, introduction, further reading and cross-references to other related encyclopedia articles. Written at a level suitable for university undergraduates, the breadth and depth of coverage will appeal beyond undergraduates to professionals and academics in related fields. 16 separate areas of microbiology covered for breadth and depth of content Extensive use of figures, tables, and color illustrations and photographs Language is accessible for undergraduates, depth appropriate for scientists Links to original journal articles via Crossref 30% NEW articles and 4-color throughout – NEW!
caused by multiple strains growing as biofilms in infected tissues seems futile,
and possibly fraudulent. ... The problem of overt culture-negative infections has
bedeviled clinicians in orthopedic surgery, ear nose and throat (ENT), and
Author: Tom Schmidt
Category: Microbial ecology
"In 2009, the third edition of the Encyclopedia of Microbiology and the Desk Encyclopedia of Microbiology published, providing customers with a six-volume compendium and condensed reference, respectively, on the vast subject of microbiology. This derivative will compile thirty-two chapters from the original MRW relating to microbial ecology (the study of how microbes interact with each other and their environments) and present them in a single thematic volume that will appeal to researchers, technicians, and students in the environmental science and microbial ecology fields. Classic and cutting-edge entries on topics including air quality, marine habitats, food webs, and microbial adhesion will be fully updated by their original authors (when possible), providing a up-to-date and affordable option to those with focused research interests"--Provided by publisher.
Top right , As infection progresses , bacterial colonization occurs within protective
exopolysaccharide biofilm , which is especially ... tract Biofilm -Extruding
sequestrum BiofilmTreatment of Soft - Tissue Injuries The appropriate time to
obtain cultures from open wounds also is controversial . ... The most common
infecting organisms seem to be gram - negative rods , gram - positive
staphylococci , and and ...
Author: Willis Cohoon Campbell
Category: Orthopedic surgery
The most trusted and widely used resource in orthopedic surgery in an all-new four-color design, providing exceptional visual guidance on more than 1,800 operative and minimally invasive procedures. Totally revised and updated, it encompasses all of the very latest procedures, techniques, and instruments. Drs. S. Terry Canale and James H. Beaty along with 22 other authorities from the Campbell Clinic present encyclopedic, in-depth coverage of the field, organized for efficient access.
The Transferrin - Transferrin Biofilm Microzone metabolites , ester ) Corrosion
Wear Trauma Figure 5 . 6 Schematic of ... The presence of Gram - negative
bacteria alone or in mixed culture provides an increased risk of failure after
The incidence of polymicrobial infections in orthopaedic surgery is probably
underrecognized . It is clear from examining the biofilm on the implants , 33 - 35
and from other in vitro studies , that ... Other issues include how we deal with an
obviously infected patient for whom cultures are negative and how we deal with ...
Author: John L. Esterhai
Publisher: Amer Academy of Orthopaedic
This text provides a guide to understanding the mechanisms involved in the pathogenesis of muscoskeletal sepsis. It covers areas such as bone, cartilage, soft tissue, and biomaterial interaction in the face of infection.
The unique behavior and innate resistance of biofilm - enclosed microbial
surface populations suggest that therapy must ... the observed resistance of such infections to high and prolonged levels of antibiotics , which culture and
sensitivity testing has indicated should be therapeutic . ... l and others
MATERIALS AND METHODS ORGANISMS Three strains of coagulase - negative
staphylococci and three ...
This instability compromises the ability to overcome infection and to heal the
fracture. Bacteria that are attached to surfaces such as metallic fixation devices or
dead bone become resistant to the action ofantibiotics through the production of
a biofilm that forms a protective covering. ... outflow.51,52 The ability to achieve a culture-negative wound may require at least two sequential debridements.32,53
Author: James P. Stannard
Winner of First Prize in Orthopaedics and Rheumatology at the 2008 British Medical Association Medical Book Competition Winner of Association of American Publishers Best Book in Clinical Medicine, 2007This landmark multimedia reference presents the most current information on surgical treatments of a wide range of injuries, from complex pelvic and spinal trauma to periarticular fractures and knee dislocations. For each injury, the authors guide the reader from the clinical evaluation, through the decision-making process, and to the surgical procedure. Each chapter provides a thorough review of surgical anatomy, streamlined discussion of the classification of the injury, the non-operative treatments available, and the indications for surgical treatment. Concise, step-by-step descriptions of surgical techniques are supplemented by carefully edited videos of surgical procedures on four accompanying DVDs, containing over 18 hours of video footage. Highlights of the book: Tips and Tricks summarize key points of each procedure for rapid review in preparation for surgery Exam Pearls describe injuries, incidence, indications for surgery, and possible complications and are ideal for board preparation More than 1,400 photographs, radiographs, and drawings illustrate techniques New Techniques sections cover the latest innovations for surgical management A separate table of contents for the four DVDs and shaded text boxes throughout the book enable convenient book-to-video reference Highlights of the DVDs: Narrated videos of entire surgical procedures provide a window into the operating room--an invaluable resource for residents and general orthopedic surgeons who do not perform trauma procedures regularly Coverage of state-of-the-art techniques such as locked plating, minimally invasive fracture fixation, advanced intramedullary nailing strategies, staged reconstruction of periarticular fractures, new techniques for hip hemiarthroplasty, surgical management of knee dislocation, including double bundle ACL and PCL knee reconstruction, and the use of temporary postoperative hinged external fixation Each DVD has been professionally produced and features high-quality audio, special effects, and multiple camera angles for each procedure Authoritative and comprehensive in its scope, this landmark text will be an invaluable asset in libraries of all orthopedic surgeons, residents, or specialists seeking a comprehensive and didactic reference.
An assay of Staphylococcus epidermidis biofilm responses to therapeutic agents
... deep sections of drivelines are culture positive and intervening sections are
lightly colonized or culture negative ( 9 ) . ... sources of infection will no doubt be
identified just as they have been for prosthetic heart valves , orthopedic implants
Author: Orthopaedic Research Society. MeetingPublish On: 2004
These CNSs are often antibiotic - resistant and may produce biofilms , making
treatment difficult . An optimal diagnosis of infection is essential , but conventional cultures sometimes show false negative results . An alternative to conventional ...
Author: Orthopaedic Research Society. Meeting
Consists of the transactions of the 22nd- annual meeting of the society.
Laboratory Controversies in Orthopedic Infections MICROORGANISMS
ENCOUNTERED IN ORTHOPEDIC ... up broth culture from a surgical wound site
, particularly when the isolate is Staphylococcus ( coagulase - negative ) that was
not seen ... 31 , 38 The second controversy relates to the role of slime or
glycocalyx biofilm that is produced by bacteria on synthetic materials used in orthopedic surgery .
Author: Ramon B. Gustilo
Publisher: W B Saunders Company
A definitive reference work for orthopaedic residents and practitioners covering all diagnostic and treatment considerations pertaining to osetomyelitic and associated infections. Addresses accepted concepts of prevention as well as long term management of infections, and discusses sources of infection such as joint replacements and newer biomaterials. DRG implication and use of the clinical laboratory are also emphasized as they relate to treatment choices.
... the ganisms are more resistant to antibiotics coagulase negative staphylococci
are fre than are planktonic forms because of phequently recovered from blood cultures of notypic changes rather than simple biofilm asymptomatic persons .
In D'Ambrosia RD , Marion RL , eds : Orthopaedic infections , Thorofare , NJ ,
1989 , Slack , pp 31-47 . 39. ... Lesse A , Freer C , Salata R : Oral ciprofloxacin
therapy for gram negative bacillary osteomyelitis , Am J Med 82 ( suppl 4A ) ...
Schurman DJ , Smith RL : Bacterial biofilm and infected biomaterials , prosthesis ,
and artificial organs . ... Caprioli R , Testa J , Courhoyer RW , Esposito FJ :
Prompt diagnosis of suspected osteomyelitis by utilizing percutaneous bone culture , J Foot ...
Author: David Edward Marcinko
Publisher: Mosby Incorporated
This is the most complete and up-to-date clinical reference encompassing all areas of diagnosis and treatment of the infected foot. Divided into five sections, which cover prevention, laboratory and clinical analysis, medical and surgical treatment, and other concerns. Some of these many "special chapters" include coverage of : the psychological implications for the infected patient, the medicolegal patient, OSHA standards and requirements, and new treatment modalities. The two forewords and eighteen chapters are contributed by some of the most well-known and respected specialists in the field. In addition, the chapters contain almost 200 high-quality clinical photographs and radiographs. (Midwest).
... on blood agar in the presence ( gram - negative plus lactobacilli count ) and
absence ( total count ) of vancomycin ( 2 . ... Results are expressed as 10 - x cfu
ml - ( culture ) or cm ( biofilm ) ( C . W . Keevil , in “ Recent Advances in Microbial
... 9 Urinary Catheter Infection Brooks and Keevil20 modified the chemostat biofilm model by replacing the stainless steel ... This material is increasingly
being used in orthopedic and dental implants , both for its physiological
properties and for its ...
This technique has been extrapolated from favorable orthopedic experiences
and may augment therapies for vascular graft infection . ... Oral linezolid , 600 mg
twice daily , can be used for MRSA , staphylococcal , and biofilm infections
between 3 and 4 ... either vancomycin ( 2 g ) , daptomycin ( 1 g ) , tobramycin ( 1
g ) , or gentamicin ( 1 g ) based on initial culture results . ... Initial graft bed
microbiology revealed 65 % gram - positive , 20 % gramnegative , and 15 %
mixed infections .
Author: Robert B. Rutherford
The seventh edition of Rutherford's Vascular Surgery-the most acclaimed comprehensive reference in vascular surgery-presents state-of-the-art updates on every aspect of vascular health care.This revision incorporates endovascular and nonoperative treatment throughout the book. It has been extensively revised by many new, international authors-led by Drs. Jack Cronenwett and K. Wayne Johnston-to meet the needs of surgeons, interventionalists, and vascular medicine specialists. Published in association with the Society for Vascular Surgery, it includes searchable online access to the full text, images, and videos through Expert Consult. Regular updates will be made to the online version based on key publications. Features all vascular imaging techniques, offering a non-invasive evaluation of both the morphology and hemodynamics of the vascular system. Discusses operative, endovascular, and non-operative management of vascular conditions to help you choose the right option for each patient. Covers the continuum of surgical management options through integrated coverage of endovascular techniques so you have the comprehensive coverage you need. Explores advances in basic science-including vessel walls, coagulation, and more-to provide the necessary background for understanding areas where fundamental changes are taking place. Published in Association with the Society for Vascular Surgery, reinforcing the clinical importance and pivotal role of this title. Provides sections dedicated to Fundamental Considerations, Patient Evaluation, Atherosclerotic Risk Factors, Perioperative Care, Bleeding and Clotting, Complications, Venous Disease, Lymphedema, Arteriovenous Anomalies, Hemodialysis Access, Miscellaneous, Technique, Grafts and Devices, Cerebrovascular Disease, Lower Extremity Arterial Disease, Upper Extremity Arterial Disease, Arterial Aneurysms, Renal and Mesenteric Disease, and Trauma and Acute Limb Ischemia. Reviews all major areas of vascular health care, written by international experts, with up to date bibliographies and annotated recommended references. Covers endovascular applications throughout the text, along with fresh approaches to many areas-including vascular access, imaging, and non-operative management-to keep you current with the latest developments and techniques. Presents a new full-color layout and design with more full-color images to give the book a modern look and feel and clarify clinical and physical findings and operative techniques. Includes access to the full text online with regular content updates, videos of procedures, an image library, and more so that you stay current and master new procedures in this fast-changing field. Your purchase entitles you to access the web site until the next edition is published, or until the current edition is no longer offered for sale by Elsevier, whichever occurs first. Elsevier reserves the right to offer a suitable replacement product (such as a downloadable or CD-ROM-based electronic version) should access to the web site be discontinued.
Author: British Biofilm Club. MeetingPublish On: 1997
Community Interactions and Control : Contributions Made at the Third Meeting of
the British Biofilm Club Held at Gregynog ... Bacteria - laden biofilms : A hazard to orthopedic prosthesis . Infect Surg 3 , 655 - 662 . Gristina , A . G . ( 1987 )
Biomaterial - centered infection : microbial adhesion versus tissue integration . ...
The isolation and culture of cells from explants of human trabecular bone . ...
Adhesion of coagulase - negative staphylococci to methacrylate polymers and
19 Special serologic tests , stains , cultures , and / or animal inoculations may be
needed . These cases need to ... 7 , 19 , 20 It may be helpful in cases in which
special stains are negative to request that surgical pathology do additional cuts
on the tissue block . The low ... Stains and cultures for mycobacteria and fungi
should be done routinely on chronic joint infections . In addition ... 25 - 27 These
organisms initiate the formation of the biofilm by adhering to surfaces via pili
There is a strong association of postoperative urinary tract infections with
prosthetic joint infection, but the significance of ... Aspiration or biopsy of a
suspected orthopedic surgical site infection is the gold standard diagnostic test. ...
False negative cultures are frequently associated with administration of
antibiotics, which should be held for at least 2 weeks prior to ... Moreover, the biofilm layer that forms within days of acute infection and is associated with many
bacteria is usually not ...
Author: Sylvia C. McKean
Publisher: McGraw Hill Professional
The leading textbook of hospital medicine – completely updated to reflect today’s challenges Since its publication in 2012, Principles and Practice of Hospital Medicine, Second Edition has become the field’s premier resource. Comprehensive, authoritative, and practical, this landmark text provides a solid grounding in clinical, organizational, and administrative areas central to the practice of hospital medicine. The Second Edition has been completely updated to reflect the evolving practice responsibilities of hospitalists. Examples include value-based medicine, expanded surgical content, bedside clinical reasoning, and a new segment devoted to rehabilitation and skilled nursing care. This edition also features a more accessible and streamlined full-color design enriched by more than 600 illustrations. Each clinical chapter opens with boxed Key Clinical Questions that are addressed in the text and summarized in hundreds of tables. Case studies demonstrate how to apply this information specifically to the management of hospitalized patients. Representing the expertise of more than two hundred renowned contributors, Principles and Practice of Hospital Medicine, Second Edition is logically divided into six sections: • The Specialty of Hospital Medicine and Systems of Care • Medical Consultation • Rehabilitation and Skilled Nursing Care • The Approach to the Patient at the Bedside • Diagnostic Testing and Procedures • Clinical Conditions in the Inpatient Setting Principles and Practice of Hospital Medicine, Second Edition is essential reading for clinicians who strive to optimize inpatient care and sharpen their leadership skills.
The latter two cultures are especially important on the pathogenesis (
hematogenous , traumatic , contiguin ... aeruginosa or other gramprovided the
pathogen is susceptible to the eluted antinegative organisms may be responsible
for the infection biotic . ... In patients with distal infection despite antibiotic therapy
, because pathogens third tibial defects , a free muscle flap is necessary . form biofilms on ...
Author: Alexander R. Vaccaro
Publisher: Amer Academy of Orthopaedic
OKU 8 is the culmination of work by a virtual 'dream team' of world-renowned orthopaedic surgeons. They selected, reviewed, and condensed the finest orthopaedic literature from 2001 - 2004 into a convenient digest you can trust and use in daily clinical decision-making. Includes annotations of approximately 2,000 source articles. OKU is your best single source for comprehensive review and thorough preparation for Board certification or recertification.