It is a key component of most chemical engineering/biotechnology/bioprocess engineering programmes. This book discusses the underlying principles of bioseparations engineering written from the perspective of an undergraduate course.
Author: Raja Ghosh
Publisher: World Scientific Publishing Company
Category: Technology & Engineering
Bioseparations engineering deals with the scientific and engineering principles involved in large-scale separation and purification of biological products. It is a key component of most chemical engineering/biotechnology/bioprocess engineering programmes. This book discusses the underlying principles of bioseparations engineering written from the perspective of an undergraduate course. It covers membrane based bioseparations in much more detail than some of the other books on bioseparations engineering. Based largely on the lecture notes the author developed to teach the course, this book is especially suitable for use as an undergraduate level textbook, as most other textbooks are targeted at graduate students.
A result of teaching and developing the subject matter over ten years, Bioseparations Engineering is an ideal text for graduate students, as well as a timely desk book for process engineers, process scientists, researchers, and research ...
Author: Michael R. Ladisch
Multidisciplinary resource for graduate studies and the biotechnology industry Knowledge of the genetic basis of biological functioning continues to grow at an astronomical rate, as do the challenges and opportunities of applying this information to the production of therapeutic compounds, specialty biochemicals, functional food ingredients, environmentally friendly biocatalysts, and new bioproducts from renewable resources. While genetic engineering of living organisms transforms the science of genomics into treatments for cancer, diabetes, and heart disease, or products for industry and agriculture, the science and technology of bioseparations are the keys to delivering these products in a purified form suitable for use by people. The methods, theory, and materials that reduce the science of bioseparations to practice, whether in the laboratory or the plant, are the subjects of Bioseparations Engineering. Examples address purification of biomolecules ranging from recombinant proteins to gene therapy products, with footnotes detailing economics of the products. Mechanistic analysis and engineering design methods are given for: * Isocratic and gradient chromatography * Sedimentation, centrifugation, and filtration * Membrane systems * Precipitation and crystallization Topics addressed within this framework are: stationary phase selection; separations development; modeling of ion exchange, size exclusion, reversed phase, hydrophobic interaction, and affinity chromatography; the impact of regulatory issues on chromatography process design; organization of separation strategies into logical sequences of purification steps; and bridges between molecular biology, combinatorial methods, and separations science. A result of teaching and developing the subject matter over ten years, Bioseparations Engineering is an ideal text for graduate students, as well as a timely desk book for process engineers, process scientists, researchers, and research associates in the pharmaceutical, food, and life sciences industries.
The first electrophoresis equipment for bioseparation purposes was invented by
the American geneticist Oliver Smithies in the mid 19th century, for aiding his
molecular studies. Recent developments in the bioseparations engineering show
Author: Ajay Kumar
Publisher: I. K. International Pvt Ltd
Category: Biochemical engineering
Bioseparation Engineering is meant for undergraduate and the postgraduate student community pursuing careers in Life Sciences. It concentrates on the more recent methods and techniques for separating components and products of the biotechnology industry. Each chapter deals with a specific type or area of application and includes information on the basic principles, industrial equipment available, commercial applications and an overview of current research and development. Main objective of the book is to provide in-depth knowledge of the subject in an interesting and paramount simple way
1.8 INTRODUCTION TO BIOSEPARATIONS: ENGINEERING ANALYSIS Modern
biotechnology is built on the genetic manipulation of organisms, genetic
engineering, to produce commercial products or processes. The implementation
Author: Roger G. Harrison
Publisher: Oxford University Press
Category: Technology & Engineering
Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field of bioseparations. Current, comprehensive, and concise, it covers bioseparations unit operations in unprecedented depth. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scaleup. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simular, SuperPro Designer® is used to analyze and evaluate the production of three important biological products. New to this second edition are updated discussions of moment analysis, computer simulation, membrane chromatography, and evaporation, among others, as well as revised problem sets. Unique features include basic information about bioproducts and engineering analysis and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals working in or studying bioseparations, and is the premier text in the field.
Author: LAURENCE R. WEATHERLEYPublish On: 2013-10-22
There are also a number of fundamental challenges and difficulties which
biotechnological processes present to the engineer and many of these impinge
directly upon separation process requirements. Most bioreactor product streams
Author: LAURENCE R. WEATHERLEY
Category: Technology & Engineering
The use of biotechnology in chemical synthesis offers up numerous advantages to the engineer in the process industries, but it also presents a number of fundamental challenges and difficulties which impinge directly on separation process requirements. The use of biochemical separations has grown significantly during the past decade, and is especially used in process industries such as healthcare and food processing. However it is becoming increasingly more important in areas such as recycling and waste-water treatment and as industry shifts towards cleaner processes biochemical separations will continue to grow. The two main objectives of this book are to focus on the application of existing separation process techniques to the recovery and purification of biologically derived products and to examine the state of knowledge of new techniques which have future potential. Within these objectives the complexities and breadth of problems associated with biological separations are discussed, specific engineering techniques are featured and their adaptation to biochemical separations are highlighted.
Bioseparation. Raja. Ghosh. CONTENTS 18.1 Bioseparation . ... 511 18.1
BIOSEPARATION Bioseparations engineering deals with the large-scale
purification of biological products such as proteins that are used as biochemicals,
Author: Anil Kumar Pabby
Publisher: CRC Press
The Handbook of Membrane Separations: Chemical, Pharmaceutical, and Biotechnological Applications provides detailed information on membrane separation technologies as they have evolved over the past decades. To provide a basic understanding of membrane technology, this book documents the developments dealing with these technologies. It explo
The scope of this book is based on the conference, and deals not only with recent advances in bioseparation engineering in a narrow sence, but also the environmental engineering which includes waste water treatment and bioremediation.
Author: I. Endo
The bioseparation engineering of today includes downstream process engineering such as waste water, material and gas treatment. Taking this tendency into account, bioseparation engineers gathered in Japan as a special research group under the main theme of "Recovery and Recycle of Resources to Protect the Global Environment". The scope of this book is based on the conference, and deals not only with recent advances in bioseparation engineering in a narrow sence, but also the environmental engineering which includes waste water treatment and bioremediation. The contributors of this book cover many disciplines such as chemical engineering, analytical chemistry, biochemistry, and microbiology. Bioseparation Engineering will stimulate young engineers and scientists who will develop bioseparation engineering further in the 21st century, and contribute to a world-wide attention to the global environment
... several technology initiatives at Amgen, and authored numerous publications
and presentations in bioseparations. ... He received his Ph.D. in chemical engineering from Rensselaer Polytechnic Institute, Troy, New York and his B.S.
Author: Abhinav A. Shukla
Publisher: CRC Press
The biopharmaceutical industry has become an increasingly important player in the global economy, and the success of these products depends on the development and implementation of cost-effective, robust and scaleable production processes. Bioseparations-also called downstream processing- can be a key source of competitive advantageto biopharmaceutical developers. Process Scale Bioseparations for the Biopharmaceutical Industry brings together scientific principles, empirical approaches, and practical considerations for designing industrial downstream bioprocesses for various classes of biomolecules. Using clear language along with numerous case studies, examples, tables, flow charts, and schematics, the book presents perspectives from experienced professionals involved in purification processes and industrial downstream unit operations. The authors provide useful experimental design strategies and guidelines for developing application-specific process scale bioseparations. Chapter topics include harvest by centrifugation and filtration, expanded bed chromatography, protein refolding, modes of preparative chromatography, methodologies for resin screening, membrane chromatography, protein crystallization, viral filtration, ultrafiltration/diafiltration, implementing post-approval downstream process changes for an antibody product, and future trends. Ideal for both new and experienced scientists in the biopharmaceutical industry and students, Process Scale Bioseparations for the Biopharmaceutical Industry is a comprehensive resource for all topics relevant to industrial process development.
Chemical Engineering Essentials Essentials of Chemical Engineering for Non - Engineers CH710 3 Days , 2 . ... each bioseparation unit operation , which will
lead to correct decisions during bioseparations process troubleshooting and
237. A. McPherson: Crystallization of Biological Macromolecules, Cold Spring
Harbor Laboratory Press, New York 1998. 238. M. R. Ladisch: Bioseparations Engineering, Wiley-Interscience, New York 2000. 239. P. M. Tessier, A. M.
Author: Wiley-VCH Staff
The one-stop resource for all those involved in the biochemical and biotechnological industries. Based on the latest online edition of Ullmann's Encyclopedia of Industrial Chemistry containing articles never seen before in print, this ready reference meets the need for a detailed survey of the biochemical fundamentals and techniques as well as their applications in biochemical engineering and biobased production.
00 This bioseparations engineering work by Ghosh ( McMaster Univ . , Canada ) ,
who teaches this subject , is one of only a ... many professionals will find it useful
to support their work as they design , build , and use bioseparation processes .
Quantitative engineering aspects of industrial applications of biology including
the microbial synthesis of commercial products ... Principles of bioseparations engineering , including specialized unit operations not normally covered in
THE STATE UNIVERSITY OF NEW JERSEY RUTGERS Graduate Program in
Chemical & Biochemical Engineering 20 ... Nucleic acid biotechnology ,
molecular biophysics and bioengineering , bioseparations Carlos B . Rosas ,
Recommended Textbooks and References for Further Reading Biochemical
Engineering Aiba , S . , Humphrey , A . E . and Millis , N . F . ( 1973 ) ... Ladisch ,
M . R . ( 2001 ) Bioseparations Engineering : Principles , Practice and Economics
Ladisch, M. R., Bioseparations Engineering—Principles, Practice, and
Economics, WileyInterscience, New York, 2001. Lee, M. N. Y., “Novel Separation
with Molecular Sieve Adsorption,” in N. N. Li (Ed.), Recent Developments in
Author: Phillip C. Wankat
Publisher: Prentice Hall
Category: Technology & Engineering
The Definitive, Fully Updated Guide to Separation Process Engineering–Now with a Thorough Introduction to Mass Transfer Analysis Separation Process Engineering, Third Edition, is the most comprehensive, accessible guide available on modern separation processes and the fundamentals of mass transfer. Phillip C. Wankat teaches each key concept through detailed, realistic examples using real data–including up-to-date simulation practice and new spreadsheet-based exercises. Wankat thoroughly covers each of today's leading approaches, including flash, column, and batch distillation; exact calculations and shortcut methods for multicomponent distillation; staged and packed column design; absorption; stripping; and more. In this edition, he also presents the latest design methods for liquid-liquid extraction. This edition contains the most detailed coverage available of membrane separations and of sorption separations (adsorption, chromatography, and ion exchange). Updated with new techniques and references throughout, Separation Process Engineering, Third Edition, also contains more than 300 new homework problems, each tested in the author's Purdue University classes. Coverage includes Modular, up-to-date process simulation examples and homework problems, based on Aspen Plus and easily adaptable to any simulator Extensive new coverage of mass transfer and diffusion, including both Fickian and Maxwell-Stefan approaches Detailed discussions of liquid-liquid extraction, including McCabe-Thiele, triangle and computer simulation analyses; mixer-settler design; Karr columns; and related mass transfer analyses Thorough introductions to adsorption, chromatography, and ion exchange–designed to prepare students for advanced work in these areas Complete coverage of membrane separations, including gas permeation, reverse osmosis, ultrafiltration, pervaporation, and key applications A full chapter on economics and energy conservation in distillation Excel spreadsheets offering additional practice with problems in distillation, diffusion, mass transfer, and membrane separation
... Ion Exchange Chromatography , " Bioseparations Engineering : Principles ,
Practice , and Economics , pp . 452 - 513 , Wiley , New York , U . S . A . ( 2001 )
Regnier , F . E . ; “ High - Performance Ion - exchange Chromatography , "
505 , Marcel Dekker , Inc. ( 1996 ) 25 Blanch , H. and Clark , D. , Biochemical Engineering , Chapter 6 , Marcel Dekker , Inc. ( 1996 ) 26 Belter P. , Cussler E. ,
and Hu W - S . Bioseparations : Downstream Processing for Biotechnology ,
This section also provides significant information for process-scale separations.
Plant and process equipment, engineering process control of bioseparation
processes, economic considerations, and future developments are discussed
Author: Satinder Ahuja
It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories. Key Features * Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies * Encompasses both analytical and process-scale methods * Elucidates the importance of engineering process control * Details selection of plant and process equipment * Addresses economic considerations * Discusses future developments
In these engineering models, elementary models of protein and ion mobility are
used, and electrical fields are usually assumed to be uniform (Gobie and Ivory,
1988; Burgaud et al., 1992) except when conductivity gradients lead to band ...
Publisher: CRC Press
Category: Technology & Engineering
Offers in-depth coverage of the latest advances in new and traditional separation technologies as they are used in a variety of ways to produce value-added products. Examines both fundamental and applied aspects of separation techniques.
BIOSEPARATION. TECHNIQUES. I. INTRODUCTION Advances in genetic engineering, rDNA technology, cell fusion techniques, and biotechnology, in
general, have made it possible to produce proteins, pharmaceuticals, drugs,
Author: Ajit Sadana
This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation. Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations. Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures. Key Features * Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include: * detailed data on biological stability under various bioseparation conditions * extensive case studies from the literature on separation processes, validation, and economics * simplified analysis of protein refolding and inactivation mechanisms * consideration of adsorption theories and the effect of heterogeneity * coverage of both classical and novel bioseparation techniques, including chromatographic procedures