This book provides the concise descriptions of the basic and clinical knowledge about Acanthamoeba keratitis, including characteristics of pathogen, risk factors, clinical manifestations, diagnosis and treatment with abundant figure ...
Author: Xuguang Sun
This book provides the concise descriptions of the basic and clinical knowledge about Acanthamoeba keratitis, including characteristics of pathogen, risk factors, clinical manifestations, diagnosis and treatment with abundant figure illustrations and typical cases to ophthalmological practitioners and researchers. Acanthamoeba pathogen is widely distributed in the nature. However, Acanthamoeba keratitis is generally considered as a type of sight-threatening keratitis that is difficult to treat. At early stage Acanthamoeba keratitis usually shows atypical clinical manifestations, that are often misdiagnosed as viral or bacterial keratitis. Moreover, there are not approved topical anti-amoebicdrugs available up to now. We hope ophthalmological practitioners can obtain a co
Abstract: Acanthamoeba is a ubiquitous opportunistically pathogenic organism that can cause a severe ocular infection (Acanthamoeba keratitis) and a fatal infection of the central nervous system (granulomatous amoebic encephalitis).
Author: Shankar Narayanan
Abstract: Acanthamoeba is a ubiquitous opportunistically pathogenic organism that can cause a severe ocular infection (Acanthamoeba keratitis) and a fatal infection of the central nervous system (granulomatous amoebic encephalitis). Acanthamoeba is thought to bind to host cells via the mannose binding protein (MBP) and produce a potent cytopathic effect (CPE) which leads to targeted cell death. Recent studies have shown that Acanthamoeba's pathogenic potential directly correlates with MBP expression and that MBP has the potential to serve as a marker for pathogenicity, however the mechanism of variable pathogenicity in Acanthamoeba isolates is still unknown. We hypothesize that the differences in pathogenicity of Acanthamoeba in humans is due to differences in MBP sequence between isolates of Acanthamoeba and that the capacity of Acanthamoeba to bind to human cells may be the main factor in its ability to initiate infection. Different isolates of Acanthamoeba were cultured, the DNA extracted, and partial MBP gene was amplified using PCR. The MBP amplimers were then sequenced. Analysis of the DNA sequences of the MBP of Acanthamoeba revealed differences between the DNA sequences of MBP between different isolates of Acanthamoeba. The results suggest that differences within the mannose binding protein may lead to different levels of pathogenicity within Acanthamoeba. Future research will be geared towards understanding how MBP is related to the cytotoxic effect as well as determining if MBP is the sole contributor to Acanthamoeba's ability to bind to cells.
Here is the premiere title in a new series presenting selected expert coverage in key indexing areas. Volume 1 includes top tips and advice on indexing philosophy (Carol Roberts), theology (Kate Mertes), biography (Martin L. White), horticulture (Thirhse Shere with Lina B. Burton), art (Susan DeRenne Coerr), encyclopedias (Marion Lerner-Levine), computer manuals (Beth Palmer), and Web sites (Fred Brown).
Author: Xuguang Sun (Ophthalmologist)Publish On: 2018
This book provides the concise descriptions of the basic and clinical knowledge about Acanthamoba keratitis, including characteristics of pathogen, risk factors, clinical manifestations, diagnosis and treatment with abundant figure ...
Author: Xuguang Sun (Ophthalmologist)
This book provides the concise descriptions of the basic and clinical knowledge about Acanthamoba keratitis, including characteristics of pathogen, risk factors, clinical manifestations, diagnosis and treatment with abundant figure illustrations and typical cases to ophthalmological practitioners and researchers. Acanthamoeba pathogen is widely distributed in the nature. However, Acanthamoeba keratitis is generally considered as a type of sight-threatening keratitis that is difficult to treat. At early stage Acanthamoeba keratitis usually shows atypical clinical manifestations, that are often misdiagnosed as viral or bacterial keratitis. Moreover, there are not approved topical anti-amoebicdrugs available up to now. We hope ophthalmological practitioners can obtain a comprehensive understanding of this infection through this book. Xuguang Sun is a professor of Ophthalmology at Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
This book is a report of a systematic laboratory study with results that point to Acanthamoeba as the possible "gymn" within which Listeria trains for encounter with mammalian cells.
Author: Yakubu Nale
Publisher: LAP Lambert Academic Publishing
Acanthamoebae are primordial phagocytes which share many features with mammalian phagocytes, particularly macrophages. Studies have shown that prior adaptation to intracellular life within Acanthamoeba does provide some pathogenic bacteria with the means to infect the more evolved mammalian cells. Listeria monocytogenes, an opportunistic foodborne pathogen, is capable of growing in a variety of mammalian cells, an indication of its remarkable adaptation to intracellular survival. This however suggests previous adaptation of Listeria in an unknown eukaryotic cell in their soil habitat. This book is a report of a systematic laboratory study with results that point to Acanthamoeba as the possible "gymn" within which Listeria trains for encounter with mammalian cells. The role of survival in amoeba and its impact on persistence of Listeria in form of biofilms and resistance to biocides were discussed. The book is a good practical guide for researchers studying bacterial-protozoa interactions.
22 Driebe WT, Stern GA, Epstein RJ, Visvesvara GS, Adi M: KomadinaTherapie: Acanthamoeba keratitis. Potential role for topical clotrimazole in combination ...
Author: Axel Kramer
Publisher: Karger Medical and Scientific Publishers
This volume collects for the first time interdisciplinary findings in ophthalmology concerning effectiveness and indications of antiseptics for the prophylaxis and therapy of infections. The first part discusses the use of various antiseptics against colonization, contamination and infection of the eye caused by viruses, bacteria, fungus and protozoa in comparison to topical antibiotics. The spectrum of action, the risk of resistance of only microbiostatic active agents and the galenic requirements of antiseptics are included as well as the local and systemic tolerance. New data to iodophors, polyhexanide and magnesium monoperphthalate are presented, and, for the first time, microbiologic requirements of ocular antiseptics are defined. In the second part the current scientific knowledge of prophylaxis and therapeutic antisepsis is presented including Credé's prophylaxis and requirements in cornea banks. The final part is reserved for additional topics such as isolation techniques, hand hygiene, hygiene of contact lenses and microbiological diagnostics. To ophthalmologists, optometrists and opticians this book will give indispensable information on latest clinical and experimental findings in the field. It will also be essential reading to hygienists, microbiologists, infectionists, pharmacologists, pharmacists, and pediatricians interested in ophthalmologic issues.
With identical strains of Acanthamoeba isolated from this patient ' s cornea and
contact lens storage case , it is possible that contact lens case contamination
played an important role in the development of his Acanthamoeba keratitis . The
180:97 Acanthamoeba, methods of identification, 180:96 Acanthamoeba, previously genus Hartmanella, 180:95 Acanthamoeba resistance to water treatments, ...
Author: George Ware
Publisher: Springer Science & Business Media
Reviews of Environmental Contamination and Toxicology attempts to provide concise, critical reviews of timely advances, philosophy, and significant areas of accomplished or needed endeavor in the total field of xenobiotics in any segment of the environment, as well as toxicological implications.
In axenic liquid media , Acanthamoeba grows well but Balamuthia does not .
Granulomatous Amebic Encephalitis Several species of small , free - living
amebae found in soil and water and belonging to the genus Acanthamoeba
cause human ...
Author: Thomas C. Orihel
Publisher: Amer Society of Clinical
Opportunistic pathogens in the immunocompromised and immunosuppressed, exotic food, and travel to tropical regions, have all increased the number and types of rare parasites now frequently seen in practice. Identifying these organisms can be challenging. To help accurately identify them and diagnose their related diseases, Parasites in Human Tissues provides complete microanatomic descriptions of more than 70 parasites that can appear in human tissue. More than 700 diagnostic and clinical images, almost 600 in full color, illustrate anatomic and morphologic details for each organism. Parasites in Human Tissues summarizes the biology and life histories of these organisms as well as their clinical presentation and pathology. Among the etiologic agents covered are protozoa, nematoda, trematoda, cestoda, acanthocephala, pseudoparasites, pentastoma, and arthropods. Pathologists, research scientists, and anyone working in clinical and diagnostic settings will find Parasites In Human Tissues a comprehensive and useful guide to this increasingly important issue.
Protease activities of Acanthamoeba polyphaga and Acanthamoeba castellanii. Can JMicrobiol 2006;52:16–23. 62. Yang, Z., Z. Cao, and N. Panjwani.
Author: Dongyou Liu
Publisher: CRC Press
The elucidation of DNA double helix in 1953 and the publication of DNA cloning protocol in 1973 have put wings under the sail of molecular biology, which has since quietly revolutionized many fields of biological science, including food microbiology. Exploiting the power and versatility of molecular technologies, molecular food microbiology extends and greatly improves on phenotypically based food microbiology, leading to the development of better diagnostics for foodborne infections and intoxications, and contributing to the design of more effective therapeutics and prophylaxes against foodborne diseases. Forming part of the Food Microbiology series, Molecular Food Microbiology provides a state of art coverage on molecular techniques applicable to food microbiology. While the introductory chapter contains an overview on the principles of current DNA, RNA and protein techniques and discusses their utility in helping solve practical problems that food microbiology is facing now and in the future, the remaining chapters present detailed moleuclar analyses of selective foodborne viruses, bacteria, fungi and parasites. Key Features: Contains a state of art overview on moleuclar techniques applicable to food microbiology research and development Presents in-depth molecular analysis of selective foodborne viruses, bacteria, fungi and parasites Highlights the utility of molecular tecniques for accurate diagnosis and effective control of foodborne diseases Includes expert contributions from international scientists involved in molecular food microbiology research Represents a highly informative textbook for students majoring in food, medical, and veterinary microbiology Offers a contemporary reference for scholars and educators wishing to keep abreast with the latest developments in molecular food microbiology With contributions from international scientists involved in molecular food microbiology research, this book constitutes an informative textbook for undergraduates and postgraduates majoring in food, medical, and veterinary microbiology; represents an indispensable guide for food, medical, and veterinary scientists engaged in molecular food microbiology research and development; and offers a contemporary update for scholars and educators trying to keep in touch with the latest developments in molecular food microbiology.
STUDIES ON THE NUCLEUS OF ACANTHAMOEBA SP . ( L. C. Card No. Mic 59-
4103 ) Rose Elizabeth Cerroni , Ph.D. Vanderbilt University , 1959 Supervisor :
Professor Robert J. Neff then incubated for 1/2 hour in 1 M cysteine in 1 M glycine
This compilation presents selected aspects of eye disease that is becoming the increasingly significant for human health worldwide.
Author: Lidia Chomicz
Some Acanthamoeba species are distributed in natural and man-made environments, in a wide range of soil and aquatic habitats, also in clinical settings. The amphizoic organisms can exist as facultative parasites - causative agents of serious human disease, Acanthamoeba keratitis. The vision-threatening eye disease occurring particularly in contact lens wearers is reported with increasing prevalence in different regions of the world. The amoebic keratitis is difficult to diagnose as clinical symptoms are similar to those observed in other eye diseases. Moreover, bacterial, viral, fungal, and amoebic co-infections frequently occur; also amoebae act as carriers for ~ 20 species pathogenic for humans, e.g. from Pseudomonas, Legionella, Mycobacterium and Escherichia genera; thus the corneal disease is frequently misdiagnosed. Complex etiology, late proper recognition of amoebic infections, and the exceptional resistance of Acanthamoeba cysts to chemicals are important factors influencing diagnostic and therapeutic difficulties. Surgical interventions are needed as an alternative treatment in refractory Acanthamoeba keratitis. It should be taken into consideration that the knowledge and awareness of increasing threat generated by the amphizoic amoebae are still insufficient. This compilation presents selected aspects of eye disease that is becoming the increasingly significant for human health worldwide.
results , we can not be certain that the masses identified were true Acanthamoeba . However , when the good clinical responses to the specific
antiAcanthamoeba treatments are considered , it appears that our confocal
diagnoses were ...
Acanthamoeba spp. are free-living amoebae that can be found in virtually any environmental niche, yet rarely cause disease.
Author: Darlene A. Dartt
Publisher: Academic Press
This selection of articles from the Encyclopedia of the Eye provides a comprehensive overview of immunological features, diseases and inflammation of the eye and its support structures and organs. Rather than taking an immunological focus that is strictly suitable for clinicians, the volume offers a considerable basic science background and addresses a broad range of topics - the immune system of the eye, its various disorders, mechanisms of inflammation of the eye and visual system, treatment, wound healing mechanisms, stem cells, and more. The first single volume to integrate comparative studies into a comprehensive resource on the neuroscience of ocular immunology Chapters are carefully selected from the Encyclopedia of the Eye by the world's leading vision researchers The best researchers in the field provide their conclusions in the context of the latest experimental results
D . , MIRELMAN D . 1990 : Differentiation of clinical tiation of Acanthamoeba .
Curr . Microbiol . 43 : 391 - 395 . isolates of Entamoeba histolytica by using
specific DNA KHAN N . A . , JARROLL E . L . , PAGET T . A . 2001 : Acanthprobes
We have been referred 1 proven case of acanthamoeba keratitis who failed to
respond to chlorhexidine but did respond to PHMB . Propamidine remains the
best documented treatment for this disease . We feel that there is insufficient