RNA Methodologies

Laboratory Guide for Isolation and Characterization

Author: Robert E. Farrell, Jr.

Publisher: Academic Press

ISBN: 9780080884950

Category: Science

Page: 744

View: 8763

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. * 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques useful for all labs
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RNA Methodologies

A Laboratory Guide for Isolation and Characterization

Author: Gerard Meurant

Publisher: Academic Press

ISBN: 0323137792

Category: Science

Page: 317

View: 9405

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA. * Studies cellular regulation using RNA as a parameter of Gene Expression * Offers RNA isolation strategies * Explains proper handling, storage, and manipulation of RNA
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RNA Methodologies

A Laboratory Guide for Isolation and Characterization

Author: Robert E. Farrell

Publisher: Academic Press

ISBN: 0122496965

Category: Science

Page: 767

View: 2371

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
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Epigenetic Regulation and Epigenomics

Author: Robert A. Meyers

Publisher: John Wiley & Sons

ISBN: 3527668624

Category: Science

Page: 1254

View: 8483

Epigenetics is a term in biology referring to heritable traits that do not involve changes in the underlying DNA sequence of the organism. Epigenetic traits exist on top of or in addition to the traditional molecular basis for inheritance. The "epigenome" is a parallel to the word "genome," and refers to the overall epigenetic state of a cell. Cancer and stem cell research have gradually focused attention on these genome modifications. The molecular basis of epigenetics involves modifications to DNA and the chromatin proteins that associate with it. Methylation, for example, can silence a nearby gene and seems to be involved in some cancers. Epigenetics is beginning to form and take shape as a new scientific discipline, which will have a major impact on Medicine and essentially all fields of biology. Increasingly, researchers are unearthing links between epigenetics and a number of diseases. Although in recent years cancer has been the main focus of epigenetics, recent data suggests that epigenetic plays a critical role in psychology and psychopathology. It is being realized that normal behaviors such as maternal care and pathologies such as Schizophrenia and Alzheimer's might have an epigenetic basis. It is also becoming clear that nutrition and life experiences have epigenetic consequences. Discover more online content in the Encyclopedia of Molecular Cell Biology and Molecular Medicine.
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The Molecular Biology of Neurological Disease

Butterworths International Medical Reviews

Author: Roger N. Rosenberg,A. E. Harding

Publisher: Butterworth-Heinemann

ISBN: 148316330X

Category: Health & Fitness

Page: 276

View: 7250

The Molecular Biology of Neurological Disease reviews advances that have been made in understanding the molecular mechanisms of neurological disorders as well as immediate and future applications of molecular biological techniques to clinical practice. This book explores the molecular genetics of neurological disease such as muscular dystrophy, Joseph disease, and Huntington's disease, along with the mitochondrial genes implicated in such conditions. This text is comprised of 18 chapters and begins by introducing the reader to the basic principles and methods of molecular genetic techniques used in the diagnosis of neurological disease. Attention then turns to several aspects of genetic expression in the brain, including the extent to which the genome is expressed in the brain. The next chapter focuses on the visualization of polyadenylated messenger RNAs in individual cells in mammalian brain using in situ hybridization techniques, combined with immunohistochemical localization of specific proteins and neuropeptides implicated in diseases such as Alzheimer dementia. This book also discusses the molecular biology of chemical synaptic neurotransmission; proteins involved in the regulation of nervous system development; and gene expression in skeletal muscle. This text then concludes with a summary of the ""neurological gene map"" as it stands in the latter part of 1987. This book is intended for physicians who grapple with the problems of neurological disorders on a daily basis, including neurologists, neurologists in training, and those in related fields such as neurosurgery, internal medicine, psychiatry, and rehabilitation medicine.
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Trends in RNA Research

Author: Peter A. McNamara

Publisher: Nova Publishers

ISBN: 9781594545061

Category: Science

Page: 315

View: 7480

RNA is a chemical found in the nucleus and cytoplasm of cells. RNA plays an important role in protein synthesis and other chemical activities of the cell. The structure of RNA is similar to that of DNA, although RNA is single stranded whereas DNA has a characteristic "double helix". Genetic information is stored by DNA in the nucleus of cells, and RNA carries that information to other parts of the cell where it is converted into protein. Three types of RNA are; mRNA (messenger RNA, which contains the specific sequence of nucleotides necessary to dictate amino acid sequence in proteins), tRNA (transfer RNA, which serves as the "adapter" to position the appropriate amino acid next to a growing polypeptide chain during protein synthesis), and rRNA (ribosomal RNA, which is the RNA component of ribosomes). This book presents state-of-the-research from throughout the world.
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RNA-Ligand Interactions, Part B: Molecular Biology Methods

Author: N.A

Publisher: Elsevier

ISBN: 008049675X

Category: Science

Page: 539

View: 7474

RNA-Ligand Interactions, Part B focuses on molecular biology methods. Major topics covered include: solution probe methods, tethered-probe methodologies, in vitro affinity selection methodologies, genetic methodologies for detecting RNA-protein interactions, protein engineering methodologies useful for RNA-protein interaction studies, and cell biology methods. RNA-Ligand Interactions, Part A, its companion, VOLUME 317 focuses on structural biology methods. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
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RNA-seq Data Analysis

A Practical Approach

Author: Eija Korpelainen,Jarno Tuimala,Panu Somervuo,Mikael Huss,Garry Wong

Publisher: CRC Press

ISBN: 1466595019

Category: Mathematics

Page: 322

View: 4220

The State of the Art in Transcriptome Analysis RNA sequencing (RNA-seq) data offers unprecedented information about the transcriptome, but harnessing this information with bioinformatics tools is typically a bottleneck. RNA-seq Data Analysis: A Practical Approach enables researchers to examine differential expression at gene, exon, and transcript levels and to discover novel genes, transcripts, and whole transcriptomes. Balanced Coverage of Theory and Practice Each chapter starts with theoretical background, followed by descriptions of relevant analysis tools and practical examples. Accessible to both bioinformaticians and nonprogramming wet lab scientists, the examples illustrate the use of command-line tools, R, and other open source tools, such as the graphical Chipster software. The Tools and Methods to Get Started in Your Lab Taking readers through the whole data analysis workflow, this self-contained guide provides a detailed overview of the main RNA-seq data analysis methods and explains how to use them in practice. It is suitable for researchers from a wide variety of backgrounds, including biology, medicine, genetics, and computer science. The book can also be used in a graduate or advanced undergraduate course.
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Methodologies and Results in Grapevine Research

Author: Serge Delrot,HipĆ³lito Medrano,Etti Or,Luigi Bavaresco,Stella Grando

Publisher: Springer Science & Business Media

ISBN: 9789048192830

Category: Technology & Engineering

Page: 448

View: 609

Grapevine is a crop of major economical interest, and wine represents a multicultural heritage which has been growing since several milleniums. Yet, modern viticulture must face several challenges. Global climate has increased berry sugar content (and alcohol in the wine) whereas phenolic and aromatic ripeness are not always achieved. Water supply is becoming shorter. New varieties better adapted to new climatic conditions might have to be planted, which may affect wine typicity. Phytochemical treatments are more controlled, and the consumer pays increasing attention to environmentally safe practices. New methods reducing pesticide use, but maintaining yield and typicity, must be designed. The present book illustrates the recent progress made in ecophysiology, molecular and cell biology, and pathology of grapevine, as well as in precision viticulture and berry composition. Combination of these new tools with field observations will undoubtly make it easier to face the challenges described above. These multidisciplinary contributions will be of interest to anyone involved in grapevine and wine activities.
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Combinatorics, Algorithms, Probabilistic and Experimental Methodologies

First International Symposium, ESCAPE 2007, Hangzhou, China, April 7-9, 2007, Revised Selected Papers

Author: Bo Chen

Publisher: Springer Science & Business Media

ISBN: 3540744495

Category: Computers

Page: 530

View: 5915

The First International Symposium on Combinatorics, Algorithms, Probabilistic and Experimental Methodologies was held in Hangzhou, China, in April 2007. The symposium provided an interdisciplinary forum for researchers to share their discoveries and approaches; search for ideas, methodologies, and tool boxes; find better, faster, and more accurate solutions; and develop a research agenda of common interest. This volume constitutes the refereed post-proceedings of the symposium. Inside you'll find 46 full papers. They represent some of the most important thinking and advancements in the field. The papers address large data processing problems using different methodologies from major disciplines such as computer science, combinatorics, and statistics.
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Recombinant DNA Principles and Methodologies

Author: James Greene

Publisher: CRC Press

ISBN: 9780824799892

Category: Science

Page: 768

View: 8129

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.
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Gene Silencing by RNA Interference

Technology and Application

Author: Muhammad Sohail

Publisher: CRC Press

ISBN: 020348925X

Category: Science

Page: 400

View: 7772

Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet. This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use.
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Advancing Cryptosporidium Parvum Detection Methodologies

Author: Mark C. Jenkins

Publisher: American Water Works Association

ISBN: 9781583212660

Category: Science

Page: 68

View: 6068

This study focused on ideal methods of detecting C. parvum oocysts which give low detection limits, specific and sensitive detection in concentraded samples, and differentiation of viable from non-viable oocysts; are usable by a water treatment facility and provide quantitative results within a few hours. Potential useful methods are continuous flow centrifugation, rel-time TaqMan PCR, cell culture inoculation followed by PCR of DNA extration, and FISH.
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RNA - Ligand Interactions, Part A: Structural Biology Methods

Author: N.A

Publisher: Elsevier

ISBN: 0080496741

Category: Science

Page: 549

View: 6515

RNA-Ligand Interactions, Part A focuses on structural biology methods. Major topics covered include semisynthetic methodologies (RNA synthetic methods and derivatization of RNA); RNA structure determination (X-ray crystallography, NMR, EM); techniques for monitoring RNA conformation and dynamics (solution methods and electrophoretic and spectroscopic methods); and modeling tertiary structure: Part B, its companion Volume 318 of Methods in Enzymology, focuses on molecular biology methods. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the Series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
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Antisense and Ribozyme Methodology

Laboratory Companion

Author: Ian Gibson

Publisher: John Wiley & Sons

ISBN: 3527612521

Category: Science

Page: 92

View: 8998

Antisense and ribozymes have a relatively short yet successful history as research tools in gene expression studies, and thus are considered as having high potential reagents in treating viral infections and cancer. This laboratory companion provides detailed information on the potential, advantages and limitations of this methodology. It critically discusses potential pitfalls, presents strategies for choosing targets and delivery systems, so as to allow the selection of the optimum methodology for achieving fast and reliable experimental success with any human or other biological system. For researchers, technicians and advanced graduates in experimental medicine, molecular and cell biology.
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Post-translational Processing

A Practical Approach

Author: S. J. Higgins,B. D. Hames

Publisher: OUP Oxford

ISBN: 9780199637959

Category: Medical

Page: 338

View: 7406

Translation of mRNA by a ribosome produces a poly-peptide known as the primary transcript. Before this primary transcript is transported to its final location to perform its intended function, it must first be altered in various ways. These alterations are collectively known as post- translational modification and are vital to the correct location and functioning of proteins.
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Cell Polarity and Subcellular RNA Localization

Author: Dietmar Richter

Publisher: Springer Science & Business Media

ISBN: 9783540411420

Category: Science

Page: 221

View: 9009

Selective cytoplasmic organelle and protein targeting has long been thought to constitute the sole determinant of cell polarity and complexity. This view has been changed, however, by the discovery of differential subcellular RNA distribution patterns. Over the past decade it has become clear that selective mRNA sorting and translation contributes to an accumulation of cognate proteins at discrete cellular sites. In this book, various systems are discussed (e.g. Xenopus oocytes, mammalian brain, invertebrate nervous system) in terms of RNA trafficking, RNA-targeting sequences (cis-elements) and RNA-transporting proteins (trans-factors), activity-dependent translational regulation and the significance of the cytoskeleton for neuronal function and plasticity. It also discusses nucleocytoplasmic export of mRNA and viral RNA as another example of subcellular RNA kinesis.
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Encyclopedia of Molecular Biology and Molecular Medicine, Plasmids to Synthetic Peptide and Nonpeptide Combinatorial Libraries

Author: Robert A. Meyers

Publisher: Wiley-Blackwell

ISBN: 9783527284757

Category: Science

Page: 517

View: 9943

This six volume Encyclopedia is the most comprehensive, detailed treatment of molecular biology and molecular medicine available today! The Encyclopedia provides a single-source library of molecular genetics and the molecular basis of life, with a focus on molecular medicine. Genetic screening, gene therapy, structural biology, and the technology and findings of the Human Genome Project are discussed in detail. The articles that comprise the set are designed as self-contained treatments. Each of the nearly 300 articles begins with an outline and a key word section which includes definitions. These features assist the scientist or student who is unfamiliar with a specific subject area. A glossary of basic terms completes each volume and defines the most commonly used terms in molecular biology. Together with the introductory illustrations found in each volume, these definitions enable readers to understand articles without referring to a dictionary, textbook, or other reference.
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RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways

Author: Lynne E. Maquat,Megerditch Kiledjian

Publisher: Academic Press

ISBN: 9780080923321

Category: Science

Page: 464

View: 1384

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. * Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway * Expert researchers introduce the most advanced technologies and techniques * Offers step-by-step lab instructions, including necessary equipment and reagents
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MRNA Processing and Metabolism

Methods and Protocols

Author: Daniel R. Schoenberg

Publisher: Springer Science & Business Media

ISBN: 1592597505

Category: Science

Page: 270

View: 3986

MRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research.
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