Lab Math

A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench

Author: Dany Spencer Adams

Publisher: CSHL Press

ISBN: 9780879696344

Category: Mathematics

Page: 275

View: 5973

Work at the biology bench requires an ever-increasing knowledge of mathematical methods and formulae. In Lab Math,Dany Spencer Adams has compiled the most common mathematical concepts and methods in molecular biology, and provided clear, straightforward guidance on their application to research investigations. Subjects range from basics such as scientific notation and measuring and making solutions, to more complex activities like quantifying and designing nucleic acids and analyzing protein activity. Tips on how to present mathematical data and statistical analysis are included. A reference section features useful tables, conversion charts and “plug and chug” equations for experimental procedures. This volume is an excellent, structured source of information that in many laboratories is often scattered and informally organized.
Release

Lab Math

A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench

Author: Dany Spencer Adams

Publisher: N.A

ISBN: 9781936113712

Category: Mathematics

Page: 332

View: 6774

Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench, 2nd edition, collects in one place the numbers and equations you rely on for your experiments and use to report your data-what they mean and how to use them-as well as easy-to-follow shortcuts for making the math easier. Written in an accessible and informal style, Lab Math describes basic mathematical principles and various tasks involving numbers, including how to calibrate lab equipment, how to make solutions, and the numbers involved in various methods for quantifying DNA, RNA, and proteins, and an all-new section on quantitative polymerase chain reaction. Basic statistical ideas and methods and the proper reporting of uncertainty are described in simple-to-understand language. Also included are reference tables, charts and "plug-and-chug" equation blanks for specific experimental procedures. Since the publication of the first edition in 2003, Lab Math has become an essential math reference and teaching resource for both on-the-spot practical information and background for understanding numerical tasks. Important additions in this second edition make Lab Math an even more useful tool for every laboratory.
Release

Statistics at the Bench

A Step-by-step Handbook for Biologists

Author: Martina Bremer,Rebecca W. Doerge

Publisher: N.A

ISBN: N.A

Category: Mathematics

Page: 167

View: 314

Statistics at the Bench is a convenient bench-side companion for biologists, designed as a handy reference guide for elementary and intermediate statistical analyses. The expectations for biologists to have a more complete understanding of statistics are growing rapidly. New technologies and new areas of science, such as microarrays, next-generation sequencing, and proteomics, have dramatically increased the need for quantitative reasoning among biologists when designing experiments and interpreting results. Even the most routine informatics tools rely on statistical assumptions and methods that need to be appreciated if the scientific results are to be correct, understood, and exploited fully. This book is not a textbook. It is an essential handbook for working scientists. Statistics at the Bench provides a simple refresher for those who have forgotten what they once knew, and an overview for those wishing to use more quantitative reasoning in their research. Statistical methods, as well as guidelines for the interpretation of results, are explained using simple examples. Throughout the book, examples are accompanied by detailed Excel commands for easy reference.
Release

At the Bench

A Laboratory Navigator

Author: Kathy Barker

Publisher: CSHL Press

ISBN: 9780879697082

Category: Science

Page: 465

View: 5171

A clue hidden in a toy ship leads Tintin on a dangerous treasure hunt.
Release

Lab Ref

A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench

Author: Albert S. Mellick

Publisher: CSHL Press

ISBN: 0879698152

Category: Science

Page: 248

View: 9208

"The first Lab Ref volume compiled recipes and reference data drawn from a selection of our manuals and was intended to save time and spare frustration." ... "In the same spirit, Lab Ref 2 again assembles in one place a new selection of reference information that should maximize the volume's value in a crowded laboratory environment."--Note.
Release

Experimental Design for Biologists

Author: David J. Glass

Publisher: CSHL Press

ISBN: 0879697350

Category: Science

Page: 206

View: 9550

Experimental Design for Biologists explains how to establish the framework for an experimental project, including the effects of using a hypothesis–driven approach versus a question/answer approach, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to the negative control, the positive control, and other categories of controls which are perhaps less recognized, such as “assumption controls”, and “experimentalist controls.” Further, there are sections on establishing the experimental system, which includes performing critical “system controls”. While the book does reference the use of statistics, statistics is not the focus of this book, but rather the way the scientist should go about framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data. There is often very little formal training in this area for biologists; therefore this text serves as an essential teaching tool for understanding the theory and practice of designing a research plan.
Release

Using the Biological Literature

A Practical Guide, Fourth Edition

Author: Diane Schmidt

Publisher: CRC Press

ISBN: 146655858X

Category: Language Arts & Disciplines

Page: 412

View: 3390

The biological sciences cover a broad array of literature types, from younger fields like molecular biology with its reliance on recent journal articles, genomic databases, and protocol manuals to classic fields such as taxonomy with its scattered literature found in monographs and journals from the past three centuries. Using the Biological Literature: A Practical Guide, Fourth Edition is an annotated guide to selected resources in the biological sciences, presenting a wide-ranging list of important sources. This completely revised edition contains numerous new resources and descriptions of all entries including textbooks. The guide emphasizes current materials in the English language and includes retrospective references for historical perspective and to provide access to the taxonomic literature. It covers both print and electronic resources including monographs, journals, databases, indexes and abstracting tools, websites, and associations—providing users with listings of authoritative informational resources of both classical and recently published works. With chapters devoted to each of the main fields in the basic biological sciences, this book offers a guide to the best and most up-to-date resources in biology. It is appropriate for anyone interested in searching the biological literature, from undergraduate students to faculty, researchers, and librarians. The guide includes a supplementary website dedicated to keeping URLs of electronic and web-based resources up to date, a popular feature continued from the third edition.
Release

The AGT Cytogenetics Laboratory Manual

Author: Marilyn S. Arsham,Margaret J. Barch,Helen J. Lawce

Publisher: John Wiley & Sons

ISBN: 1119061229

Category: Science

Page: 1218

View: 4787

Cytogenetics is a segment of the evolving cytogenomic branch of genetics that explores the genetic makeup of a cell, in particular chromosomes both in metaphase and in interphase cycles, as well as at the molecular level. It utilizes an assortment of procedures to illuminate both the full complement of chromosomes and a targeted region within a specific chromosome. These investigative tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive treatment of the diagnostic tests offered by this clinical laboratory environment and explains the scientific theories behind those procedures. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters that reflect the intuitive experience of its authors. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN's cytogenetic language; tips for laboratory management; examples of laboratory information systems; a collection of Internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student with a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the multiple facets behind each of these procedures. Topic coverage is rich in detail, yet presented with the student in mind, making it a useful resource for researchers, clinicians, and laboratory professionals, as well as students in a university or medical school that offers introductory or laboratory-related courses exploring cytogenetics' role within medical genetics. Reviews for the third edition: "A collection of protocols and explanations for cytogenetic techniques from the common to the peculiar." "An excellent book. Looking forward to an updated version."
Release

Linne & Ringsrud's Clinical Laboratory Science

The Basics and Routine Techniques

Author: Mary Louise Turgeon

Publisher: Elsevier Health Sciences

ISBN: 0323370616

Category: Medical

Page: 688

View: 8960

Using a discipline-by-discipline approach, Linne & Ringsrud's Clinical Laboratory Science: Concepts, Procedures, and Clinical Applications, 7th Edition provides a fundamental overview of the skills and techniques you need to work in a clinical laboratory and perform routine clinical lab tests. Coverage of basic laboratory techniques includes key topics such as safety, measurement techniques, and quality assessment. Clear, straightforward instructions simplify lab procedures, and are described in the CLSI (Clinical and Laboratory Standards Institute) format. Written by well-known CLS educator Mary Louise Turgeon, this text includes perforated pages so you can easily detach procedure sheets and use them as a reference in the lab! Hands-on procedures guide you through the exact steps you'll perform in the lab. Review questions at the end of each chapter help you assess your understanding and identify areas requiring additional study. A broad scope makes this text an ideal introduction to clinical laboratory science at various levels, including CLS/MT, CLT/MLT, and Medical Assisting, and reflects the taxonomy levels of the CLS/MT and CLT/MLT exams. Detailed full-color illustrations show what you will see under the microscope. An Evolve companion website provides convenient online access to all of the procedures in the text, a glossary, audio glossary, and links to additional information. Case studies include critical thinking and multiple-choice questions, providing the opportunity to apply content to real-life scenarios. Learning objectives help you study more effectively and provide measurable outcomes to achieve by completing the material. Streamlined approach makes it easier to learn the most essential information on individual disciplines in clinical lab science. Experienced author, speaker, and educator Mary Lou Turgeon is well known for providing insight into the rapidly changing field of clinical laboratory science. Convenient glossary makes it easy to look up definitions without having to search through each chapter. NEW! Procedure worksheets have been added to most chapters; perforated pages make it easy for students to remove for use in the lab and for assignment of review questions as homework. NEW! Instrumentation updates show new technology being used in the lab. NEW! Additional key terms in each chapter cover need-to-know terminology. NEW! Additional tables and figures in each chapter clarify clinical lab science concepts.
Release

Measuring Shape

Author: F. Brent Neal,John C. Russ

Publisher: CRC Press

ISBN: 1351833146

Category: Computers

Page: 440

View: 8779

"John Russ is the master of explaining how image processing gets applied to real-world situations. With Brent Neal, he’s done it again in Measuring Shape, this time explaining an expanded toolbox of techniques that includes useful, state-of-the-art methods that can be applied to the broad problem of understanding, characterizing, and measuring shape. He has a gift for finding the kernel of a particular algorithm, explaining it in simple terms, then giving concrete examples that are easily understood. His perspective comes from solving real-world problems and separating out what works in practice from what is just an abstract curiosity."? —Tom Malzbender, Hewlett-Packard Laboratories, Palo Alto, California, USA Useful for those working in fields including industrial quality control, research, and security applications, Measuring Shape is a handbook for the practical application of shape measurement. Covering a wide range of shape measurements likely to be encountered in the literature and in software packages, this book presents an intentionally diverse set of examples that illustrate and enable readers to compare methods used for measurement and quantitative description of 2D and 3D shapes. It stands apart through its focus on examples and applications, which help readers quickly grasp the usefulness of presented techniques without having to approach them through the underlying mathematics. An elusive concept, shape is a principal governing factor in determining the behavior of objects and structures. Essential to recognizing and classifying objects, it is the central link in manmade and natural processes. Shape dictates everything from the stiffness of a construction beam, to the ability of a leaf to catch water, to the marketing and packaging of consumer products. This book emphasizes techniques that are quantitative and produce a meaningful yet compact set of numerical values that can be used for statistical analysis, comparison, correlation, classification, and identification. Written by two renowned authors from both industry and academia, this resource explains why users should select a particular method, rather than simply discussing how to use it. Showcasing each process in a clear, accessible, and well-organized way, they explore why a particular one might be appropriate in a given situation, yet a poor choice in another. Providing extensive examples, plus full mathematical descriptions of the various measurements involved, they detail the advantages and limitations of each method and explain the ways they can be implemented to discover important correlations between shape and object history or behavior. This uncommon assembly of information also includes sets of data on real-world objects that are used to compare the performance and utility of the various presented approaches.
Release

Calcium Techniques

A Laboratory Manual

Author: Jan B. Parys,Martin D. Bootman,David I. Yule,Geert Bultynck

Publisher: N.A

ISBN: 9781621820789

Category: Science

Page: 592

View: 1866

"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
Release

Handbook of Biological Confocal Microscopy

Author: James Pawley

Publisher: Springer Science & Business Media

ISBN: 1461571332

Category: Medical

Page: 232

View: 5078

In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.
Release

Ahead of the Curve

Hidden Breakthroughs in the Biosciences

Author: Dany Adams

Publisher: Iop Expanding Physics

ISBN: 9780750313278

Category: Science

Page: 150

View: 4331

This unique book is a compendium of carefully curated published papers in the biosciences, which have (or will) precipitate a profound change in prevailing paradigms and research programs. A mix of new and classic papers, it shows the limitations of current thought or identifies novel vistas for investigations that have not yet been explored. The purpose of the book is to highlight scientific gems, most unrecognized, that suggest revisions to key pillars of thought in the biological sciences and further the education of young scientists. This will be achieved by including reprints of papers that demonstrate counter-paradigm, novel directions for future research featuring commentary from current, notable researchers in a variety of areas.
Release

I Wish I'd Made You Angry Earlier

Essays on Science, Scientists, and Humanity

Author: Max F. Perutz

Publisher: Oxford University Press, USA

ISBN: 9780198590279

Category: Creative ability in science

Page: 354

View: 3625

This collection of essays from Nobel Laureate Max Perutz explores a wide range of scientific and personal topics with insight and lucidity. It includes lively anecdotes about key figures in 20th-century science.
Release

The Mis-education of the Negro

Author: Carter Godwin Woodson

Publisher: ReadaClassic.com

ISBN: N.A

Category: African Americans

Page: 207

View: 4853

Woodson's classic work of criticism explores how the education received by blacks has failed to give them an appreciation of themselves as a race and their contributions to history. Woodson puts forward a program that calls for the educated to learn about their past and serve the black community. (Education/Teaching)
Release

Textbook of Clinical Embryology

Author: Kevin Coward,Dagan Wells

Publisher: Cambridge University Press

ISBN: 110727625X

Category: Medical

Page: N.A

View: 4233

The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.
Release

A Bioinformatics Guide for Molecular Biologists

Author: Sarah J. Aerni,Sarah Aerni,Marina Sirota

Publisher: N.A

ISBN: 9781936113224

Category: Medical

Page: 319

View: 1458

A more complete understanding of bioinformatics offered in this title will allow the reader to become comfortable with them, encouraging their use and thus helping to make sense of the vast accumulation of data.
Release

Mouse Models of Cancer

A Laboratory Manual

Author: Cory Abate-Shen,Katerina Politi,Lewis A. Chodosh,Kenneth P. Olive

Publisher: N.A

ISBN: 9781621820031

Category: Medical

Page: 521

View: 5290

Too often both composition teachers and their students experience knowledge and authority as lying outside their rhetorical interactions with others. Drawing on feminist, cultural, and poststructuralist theory, as well as work in the rhetorical tradition and composition studies, Hill offers less debilitating methods of thinking that teachers can model for their students. Richly illustrated with examples of classroom interactions and student work, the book also shows teachers how to enrich their own intellectual and political lives within the academy.
Release

Purifying and Culturing Neural Cells

A Laboratory Manual

Author: Ben Barres,Beth Stevens

Publisher: Philip's

ISBN: 9781621820116

Category: Science

Page: 205

View: 1994

Composed of countless neurons, glia, and vascular cells, the nervous system innervates all parts of the body to function as a vast communication network. This complexity makes it challenging to examine neural properties at the cellular and molecular levels. Cell culture systems for specific neural cell types are thus essential for studies of their development and function. This laboratory manual provides step-by-step protocols for isolating specific cell populations from rodent tissues and culturing them under conditions that closely resemble those in vivo. The contributors describe in detail how to dissect the brain, spinal cord, and other tissues; how to separate cells using mechanical and enzymatic tissue-dissociation strategies; the use of immunopanning and fluorescence-activated cell sorting (FACS) to enrich the target cell population; and the culture conditions that optimize cell viability and growth. Retinal ganglion cells, motor neurons, dorsal root ganglion cells, astrocytes, oligodendrocytes, and Schwann cells are covered, as are vascular cells such as pericytes and endothelial cells. Myelinating cocultures of neurons and oligodendrocytes are also described. The manual includes detailed recipes for media and reagents, tips for avoiding common pitfalls, and advice for designing new immunopanning protocols using tissues from other sources. Many of the protocols are accompanied by freely accessible online movies that demonstrate critical steps of the procedures. This is an essential laboratory companion for all neurobiologists, from the graduate student level upward.
Release

The Labor Market for Health Workers in Africa

A New Look at the Crisis

Author: Agnes Soucat,Richard Scheffler,Tedros Ghebreyesus

Publisher: World Bank Publications

ISBN: 0821395580

Category: Business & Economics

Page: 256

View: 5560

Sub-Saharan Africa has only 12 percent of the global population, yet this region accounts for 50 percent of child deaths, more than 60 percent of maternal deaths, 85 percent of malaria cases, and close to 67 percent of people living with HIV. Sub-Saharan Africa, however, has the lowest number of health workers in the world-significantly fewer than in South Asia, which is at a comparable level of economic development. The Labor Market for Health Workers in Africa uses the analytical tools of labor markets to examine the human resource crisis in health from an economic perspective. Africa's labor markets are complex, with resources coming from governments, donors, the private sector, and households. Low numbers of health workers and poor understanding of labor market dynamics are major impediments to improving health service delivery. Yet some countries in the region have developed innovative solutions with new approaches to creating a robust health workforce that can respond to the continent's health challenges. As Africa grows economically, the invaluable lessons in this book can help build tomorrow's African health systems.
Release