Handbook of Biological Confocal Microscopy

Author: James Pawley

Publisher: Springer Science & Business Media

ISBN: 1475753489

Category: Science

Page: 632

View: 5016

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.
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Funktionelle Ultrastruktur

Atlas der Biologie und Pathologie von Geweben

Author: Margit Pavelka,Jürgen Roth

Publisher: Springer-Verlag

ISBN: 3211308261

Category: Science

Page: 334

View: 8044

Mit dem Blick auf die Feinstrukturen werden die Innenräume der Zellen und ihre Architekturen in der höheren Organisation der Gewebe lebendig. Die Autoren geben mit diesem Atlas der funktionellen Ultrastruktur Einblick in die komplexe zelluläre Innenwelt mit ihren vielgestaltigen Funktionsräumen, sowie in dynamische Funktionsabläufe, die für die Erfüllung der zellulären Aufgaben im Gewebe notwendig sind. Das besondere Anliegen besteht darin, dem Leser bewusst zu machen, dass es sich hier um diejenigen Strukturen handelt, in denen sich grundlegende zelluläre Lebensvorgänge und krankmachende Mechanismen abspielen. An Hand elektronenmikroskopischer Bilder, ergänzt durch Immunelektronenmikroskopie und ausführlicher Beschreibung werden in den Zellen die Beziehungen zwischen Strukturen und Funktionen aufgerollt, Details herausgearbeitet und die Organisation in den Zellverbänden der Gewebe präsentiert. Das Buch wendet sich an Medizin- und Naturwissenschaftsstudenten, Ärzte, Biologen, Zell- und Molekularbiologen.
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Form and Function of Mammalian Lung: Analysis by Scientific Computing

Author: Andres Kriete

Publisher: Springer Science & Business Media

ISBN: 3642722202

Category: Science

Page: 108

View: 1531

1.1 Overview The precise knowledge of the three-dimensional (3-D) assembly of biological structures is still in its origin. As an example, a widely accepted concept and common belief of the structure of the airway network oflung is that of a regular, dichotomous branching pattern, also known as the trumpet model. This model, first introduced by Weibel in 1963, is often used in clinical and physiological applications. However, if this concept of dichotomy is used to model lung, a shape is obtained that is quite different from a real lung. As a matter of fact, many previous quantitative morphological and stereological investigations of lung did not concentrate on the spatial aspect of lung morphology but delivered data in a more statistical fashion. Accordingly, the functional behavior predicted by such a model becomes questionable and indeed, the morphometrically predicted lung capacity exceeds the physiological required capacity by a factor of 1.3 up to a factor of2. This problem has also been termed a paradox, as discussed by Weibel in 1983. In the rare cases where descriptive models of the mammalian bronchial tree exist, monopodial in small mammals, dichotomous in larger ones, the understanding of the historical and/or functional reasons for size-related changes in the general design is not explainable. This investigation is trying to overcome this gap by computer modeling and functional simulation.
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Imaging Marine Life

Macrophotography and Microscopy Approaches for Marine Biology

Author: Emmanuel G. Reynaud

Publisher: John Wiley & Sons

ISBN: 3527675426

Category: Medical

Page: 280

View: 6626

Written by an international team of experts from the Tara Oceans Marine Biology Imaging Platform (TAOMI), this is the first and only compendium on marine imaging technologies, and includes all known underwater as well as on-land techniques. TAOMI is imaging the largest collection of marine organisms in recent history, ranging from viruses to corals, and is duplicated on land to perform high throughput confocal analysis of plankton, X-ray tomography as well as cryo-electron microscopy. This unique platform combines underwater imaging with cytometry, stereomicroscopy, fluorescence microscopy and 3D microscopy - all of which are covered in this practical book, along with remote sensing, MRI, and optical projection tomography. The definitive resource for every marine biologist who is planning to image marine species, whether underwater or on land.
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Video Microscopy

Author: Greenfield Sluder,David E. Wolf

Publisher: Gulf Professional Publishing

ISBN: 9780126491609

Category: Medical

Page: 334

View: 2338

Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques. Chapters cover the commonly used video technologies in biological research Nontechnical presentation of principles Emphasis on the practical aspects of instrument use Covers pitfalls in instrument uses that can lead to artifacts Authors are leaders in the design and application of video methods to biological microscopy Presentation of material tailored for the established researcher that has little experience with video methods Examples are extensively illustrated with photographs
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Medizinische Physik 3

Medizinische Laserphysik

Author: Josef F. Bille,Wolfgang C. Schlegel

Publisher: Springer-Verlag

ISBN: 3540266305

Category: Science

Page: 453

View: 8254

Die medizinische Physik hat sich in den letzten Jahren zunehmend als interdisziplinäres Gebiet profiliert. Um dem Bedarf nach systematischer Weiterbildung von Physikern, die an medizinischen Einrichtungen tätig sind, gerecht zu werden, wurde das vorliegende Werk geschaffen. Es basiert auf dem Heidelberger Kurs für medizinische Physik. Die drei Bände vermitteln das für die Fachanerkennung als Medizinphysiker notwendige medizinische und physikalische Wissen. Im Band I werden die medizinischen, medizintechnischen und biomathematischen Grundlagen behandelt. Band II ist der medizinischen Strahlenphysik, Tomographie, Ultraschalldiagnose, Nuklearmedizin und dem Strahlenschutz gewidmet. Band III stellt Grundlagen und Anwendung der medizinischen Laserphysik und Optik vor.
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Biophotonics

Author: N.A

Publisher: Elsevier

ISBN: 9780080522586

Category: Science

Page: 714

View: 8450

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today truly an essential publication for researchers in all fields of life sciences. * Discusses optical instrumentation for imaging, screening and diagnosis in molecules, tissues, and cells * Covers the development and application of optical probes and techniques for imaging and drug screening * Investigates the structure and dynamics of biomolecular systems, screening and drug discovery, and the diagnosis and treatment of disease
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Photonics, Volume 4

Biomedical Photonics, Spectroscopy, and Microscopy

Author: David L. Andrews

Publisher: John Wiley & Sons

ISBN: 1119011795

Category: Technology & Engineering

Page: 576

View: 6433

Discusses the basic physical principles underlying Biomedical Photonics, spectroscopy and microscopy This volume discusses biomedical photonics, spectroscopy and microscopy, the basic physical principles underlying the technology and its applications. The topics discussed in this volume are: Biophotonics; Fluorescence and Phosphorescence; Medical Photonics; Microscopy; Nonlinear Optics; Ophthalmic Technology; Optical Tomography; Optofluidics; Photodynamic Therapy; Image Processing; Imaging Systems; Sensors; Single Molecule Detection; Futurology in Photonics. Comprehensive and accessible coverage of the whole of modern photonics Emphasizes processes and applications that specifically exploit photon attributes of light Deals with the rapidly advancing area of modern optics Chapters are written by top scientists in their field Written for the graduate level student in physical sciences; Industrial and academic researchers in photonics, graduate students in the area; College lecturers, educators, policymakers, consultants, Scientific and technical libraries, government laboratories, NIH.
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Biological Imaging and Sensing

Author: Toshiyuki Furukawa

Publisher: Springer Science & Business Media

ISBN: 9783540438984

Category: Medical

Page: 298

View: 5677

Topics discussed in this book include: cell imaging, multiphoton microscopy for biomedical studies, molecular imaging, infrared imaging, biomedical magnetic imaging, and microscopy with laser-trapped particles. The book also deals with nanosurgery with light, the effects of ultrasound on tissue, diagnostics, near- and far-infrared transmission of biomedical information, and cell sensors.
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Manual of Environmental Microbiology

Author: Christon J. Hurst,Ronald L. Crawford,Jay L. Garland,David A. Lipson

Publisher: American Society for Microbiology Press

ISBN: 1555813798

Category: Science

Page: 1293

View: 2362

The most definitive manual of microbes in air, water, and soil and their impact on human health and welfare. • Incorporates a summary of the latest methodology used to study the activity and fate of microorganisms in various environments. • Synthesizes the latest information on the assessment of microbial presence and microbial activity in natural and artificial environments. • Features a section on biotransformation and biodegradation. • Serves as an indispensable reference for environmental microbiologists, microbial ecologists, and environmental engineers, as well as those interested in human diseases, water and wastewater treatment, and biotechnology.
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Principles of Nano-Optics

Author: Lukas Novotny,Bert Hecht

Publisher: Cambridge University Press

ISBN: 113956045X

Category: Science

Page: N.A

View: 1159

First published in 2006, this book has become the standard reference on nano-optics. Now in its second edition, the text has been thoroughly updated to take into account new developments and research directions. While the overall structure and pedagogical style of the book remain unchanged, all existing chapters have been expanded and a new chapter has been added. Adopting a broad perspective, the authors provide a detailed overview of the theoretical and experimental concepts that are needed to understand and work in nano-optics, across subfields ranging from quantum optics to biophysics. New topics of discussion include: optical antennas; new imaging techniques; Fano interference and strong coupling; reciprocity; metamaterials; and cavity optomechanics. With numerous end-of-chapter problem sets and illustrative material to expand on ideas discussed in the main text, this is an ideal textbook for graduate students entering the field. It is also a valuable reference for researchers and course teachers.
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The Chemistry of Molecular Imaging

Author: Nicholas Long,Wing-Tak Wong

Publisher: John Wiley & Sons

ISBN: 1118854810

Category: Technology & Engineering

Page: 408

View: 8317

Molecular imaging is primarily about the chemistry of novelbiological probes, yet the vast majority of practitioners are notchemists or biochemists. This is the first book, written from achemist's point of view, to address the nature of the chemicalinteraction between probe and environment to help elucidatebiochemical detail instead of bulk anatomy. Covers all of the fundamentals of modern imaging methodologies,including their techniques and application within medicine andindustry Focuses primarily on the chemistry of probes and imagingagents, and chemical methodology for labelling andbioconjugation First book to investigate the chemistry of molecularimaging Aimed at students as well as researchers involved in the areaof molecular imaging
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Microbial Extracellular Polymeric Substances

Characterization, Structure, and Function

Author: Jost Wingender,Thomas R. Neu

Publisher: Springer Science & Business Media

ISBN: 9783540657200

Category: Science

Page: 258

View: 7624

Microbial extracellular polymeric substances (EPS) are the key components for the aggregation of microorganisms in biofilms, flocs and sludge. They are composed of polysaccharides, proteins, nucleic acids, lipids and other biological macromolecules. EPS provide a highly hydrated gel matrix in which microbial cells can establish stable synergistic consortia. Cohesion and adhesion as well as morphology, structure, biological function and other properties such as mechanical stability, diffusion, sorption and optical properties of microbial aggregates are determined by the EPS matrix. Also, the protection of biofilm organisms against biocides is attributed to the EPS. Their matrix allows phase separation in biofiltration and is also important for the degradation of particulate material which is of great importance for the self purification processes in surface waters and for waste water treatment. In this volume, analysis, characterization, composition, regulation, function and interactions of microbial EPS are covered.
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Cell Biological Applications of Confocal Microscopy

Author: N.A

Publisher: Elsevier

ISBN: 9780080496580

Category: Science

Page: 507

View: 9992

This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures
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Programmieren mit R

Author: Uwe Ligges

Publisher: Springer-Verlag

ISBN: 3540267328

Category: Mathematics

Page: 237

View: 8777

R ist eine objekt-orientierte und interpretierte Sprache und Programmierumgebung für Datenanalyse und Grafik - frei erhältlich unter der GPL. Ziel dieses Buches ist es, nicht nur ausführlich in die Grundlagen der Sprache R einzuführen, sondern auch ein Verständnis der Struktur der Sprache zu vermitteln. Leicht können so eigene Methoden umgesetzt, Objektklassen definiert und ganze Pakete aus Funktionen und zugehöriger Dokumentation zusammengestellt werden. Die enormen Grafikfähigkeiten von R werden detailliert beschrieben. Das Buch richtet sich an alle, die R als flexibles Werkzeug zur Datenenalyse und -visualisierung einsetzen möchten: Studierende, die Daten in Projekten oder für ihre Diplomarbeit analysieren möchten, Forschende, die neue Methoden ausprobieren möchten, und diejenigen, die in der Wirtschaft täglich Daten aufbereiten, analysieren und anderen in komprimierter Form präsentieren.
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Ultrashort Laser Pulses in Biology and Medicine

Author: Markus Braun,Peter Gilch,Wolfgang Zinth

Publisher: Springer Science & Business Media

ISBN: 9783540735663

Category: Technology & Engineering

Page: 320

View: 1729

Learn about the many biological and medical applications of ultrashort laser pulses. The authors highlight and explain how the briefness of these laser pulses permits the tracing of even the fastest processes in photo-active bio-systems. They also present a variety of applications that rely on the high peak intensity of ultrashort laser pulses. Easy-to-follow examples cover non-linear imaging techniques, optical tomography, and laser surgery.
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