Artificial Nucleases

Author: Marina A. Zenkova

Publisher: Springer Science & Business Media

ISBN: 364218510X

Category: Science

Page: 292

View: 2176

The development of agents capable of cleaving RNA and DNA has attracted considerable attention from researchers in the last few years, because of the immediate and very important applications they can find in the emerging fields of biotechnology and pharmacology. There are essentially two classes of these agents - nucleases that occur naturally inside cells and synthetically produced artificial nucleases. The first class includes protein enzyme nucle ases and catalytic RNA structured ribozymes that perform cleavage of the phosphodiester bonds in nucleic acids according to a hydrolytic pathway in the course of different biochemical processes in the cell. A different pathway is used by some antibiotics which cleave DNA via redox-based mechanisms resulting in oxidative damage of nucleotide units and breakage of the DNA backbone. The above molecules are indispensable tools for manipulating nucleic acids and processing RNA; DNA-cleaving antibiotics and cytotoxic ribonucleases have demonstrated utility as chemotherapeutic agents. The second class, artificial nucleases, are rationally designed to imitate the active centers of natural enzymes by simple structures possessing minimal sets of the most important characteristics that are essential for catalysis. A dif ferent approach, in vitro selection, was also used to create artificial RNA and DNA enzymes capable of cleaving RNA. Being less efficient and specific as compared to the natural enzymes, the primitive mimics are smaller and robust and can function in a broad range of conditions.

Macrocyclic Polyamines

Synthesis and Applications

Author: Xiaoqi Yu,Ji Zhang

Publisher: John Wiley & Sons

ISBN: 3527341870

Category: Science

Page: 232

View: 7981

The first comprehensive book focusing on synthesis and applications of macrocyclic polyamines and their derivatives Macrocyclic polyamines are a class of widely used important compounds. This is the first book that systematically summarizes the synthesis and applications of macrocyclic polyamines and their analogues, including the properties and synthetic methods of macrocyclic polyamines, chemical nucleases based on macrocyclic polyamines, the derivatives of macrocyclic polyamines as nano-vector materials, macrocyclic polyamines derivatives for bio-imaging, chemical sensors based on macrocyclic polyamines, and other applications of macrocyclic polyamines. Macrocyclic Polyamines: Synthesis and Applications includes most of the studies involving macrocyclic polyamines and their derivatives, and may be used as a reference for the researchers in related fields. It offers in-depth coverage of cyclization modes; special procedures for tetraza macrocyclic compounds; diacids-diamines condensation; oxidative DNA cleaving by macrocyclic polyamines; lipids with cationic MPA headgroups; the derivatives of DOTA, DO3A, and PCTA; receptors for anions; sensors for bioactive molecules; macrocyclic polyamines for solvent extraction and membrane transport of amino acids and their derivatives, electrophoretic separation, and open-tubular CEC; and much more. The first book that systematically summarizes the chemistry of macrocyclic polyamines and their derivatives in terms of synthetic methods for their preparation, functionalization, and application in the main fields of chemical sensors, chemical nucleases, drug-delivery, bio-imaging and vector materials Provides a comprehensive reference for the researchers working on macrocyclic polyamines Offers train of thought in related research fields such as organic chemistry, coordination chemistry, analytical chemistry, supramolecular chemistry, biomaterials, etc. Macrocyclic Polyamines: Synthesis and Applications will not only provide a reference for the researchers working on macrocyclic polyamines, but also offer opportunities for researchers in related research fields to understand the benefits of these key compounds.

Polymers and Light

Fundamentals and Technical Applications

Author: Wolfram Schnabel

Publisher: John Wiley & Sons

ISBN: 3527611037

Category: Technology & Engineering

Page: 396

View: 1956

This first book to focus on the important and topical effect of light on polymeric materials reflects the multidisciplinary nature of the topic, building a bridge between polymer chemistry and physics, photochemistry and photophysics, and materials science. Written by one experienced author, a consistent approach is maintained throughout, covering such applications as nonlinear optical materials, core materials for optical waveguides, photoresists in the production of computer chips, photoswitches and optical memories. Advanced reading for polymer, physical and organic chemists, manufacturers of optoelectronic devices, chemical engineers, and materials scientists.

The Regulation of DNA Replication and Transcription

Author: Mirko Beljanski, PhD

Publisher: Demos Medical Publishing

ISBN: 1617051829

Category: Medical

Page: 160

View: 1522

The Regulation of DNA Replication and Transcription explores basic processes of DNA replication and transcription in an effort to identify the mechanisms responsible for the release of genetic information and its role in the regulation of cellular events. Concerned with discovering the fundamental concept that might integrate and explain the wide range of existing lines of evidence, the author reports and interprets the results of experiments conducted in an impressive range of biological systems. Focused on complex mechanisms at the biochemical level, these studies allow analysis of the pathways involved when cells, organs and animal systems react to various trigger molecules derived from both living cells and exogenous sources. These include hormones, RNA, RNA fragments, alkaloids, actinomycin D, and phorbol esters, as well as chemical carcinogens and drugs. Comnining the results of these studies with his own extensive work in this field, the author is able to formulate a uniquely integrative biochemical model for the gene expression, demonstrating that both biological and chemically synthesized molecules can trigger the differential release of information from the DNA and thus influence cell transformation. Apart from its academic significance, the model offers high potential assistance in the search for ways to induce or control the expression of certain genes and, moreover, to promote differentiation of given cells in vitro as well as in situ.

Molecular Biology

Author: N.A

Publisher: N.A


Category: Biology

Page: N.A

View: 2843


Molekulare Biotechnologie

Grundlagen und Anwendungen

Author: David Clark,Nanette Pazdernik

Publisher: Springer-Verlag

ISBN: 3827421896

Category: Science

Page: 707

View: 5374

Grundlage aller biotechnologischen Prozesse sind molekularbiologische und genetische Regelmechanismen. Deshalb behandelt dieses neuartige Lehrbuch beides: die molekularbiologischen Grundlagen und die Anwendungen. Spannend und aktuell werden die Teilgebiete der Biotechnologie und das jeweils erforderliche molekularbiologische Grundwissen beschrieben. Der Bogen wird gespannt von der Nanobiotechnologie über Stoffwechseltechnologie, Genomics und Umweltbiotechnologie bis hin zur Gentherapie.

Medizinische Mikrobiologie

Author: Ernest Jawetz,Joseph L. Melnick,Edward A. Adelberg

Publisher: Springer-Verlag

ISBN: 3662001551

Category: Science

Page: N.A

View: 795

Die Autoren haben beabsichtigt, diejenigen Gebiete der medizinischen Mikrobiologie kurz, exakt und in ihrem gegenwärtigen Stand darzustellen, die für die Infektionskrankheiten und ihre Chemotherapie von besonderer Bedeutung sind. Das Buch wendet sich in erster Linie an Medizinstudenten, außerdem an alle Ärzte in Krankenhaus und Praxis. Die Notwendigkeit für ein klares Verständnis der mlkrobiologischen Grundtatsachen ist in den letzten Jahren stärker geworden, da in Biochemie, Virologie und Chemotherapie so wie auf weiteren, die Medizin beeinflussenden Gebieten bedeutende Entwicklungen statt gefunden haben. Ein wesendicher Teil dieses Lehrbuches ist deswegen der Darstellung der Grundlagen gewidmet, wodurch sich das Buch auch für die Einführung des Studenten in den mikrobiologischen Kurs als brauchbar erweisen wird. Für die neue Auflage wurde ein Kapitel über Virus und Krebs neu geschrieben, und das Kapitel über antimikrobielle Chemotherapie wurde von Grund auf neu bearbeitet. Die Autoren freuen sich, bei dem Erscheinen der 7. amerikanischen Ausgabe von einer spanischen, deutschen, italienischen und griechischen Ausgabe, sowie von der Vorbereitung für eine serbokroatische Übersetzung berichten zu können. Die Autoren möchten nochmals allen danken, die ihnen bei der Bearbeitung dieser Auf lage behilflich waren, und die es ihnen durch ihre Kommentare und ihre Kritik ermöglicht haben, die in 2jährigen Abständen erscheinenden Revisionen dieses Buches auf dem neu esten Stand zu halten. ERNEsr JAWETZ JOSEPH L MELNICK San Frandsco, August 1966 EnWARD A AnELBERG III Inhaltsverzeichnis 1 Kapitell Die ~lt der Mikroben 8 Kapitel 2 Cytologie der Bakterien Optische Methoden 8 9 Eukaryote Zellstrukturen Prokaryote Zellstrukturen . 12 14 Cytoplasmatische Strukturen. 27 Färbeverfahren .